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重要文件

L3289

Sigma-Aldrich

血液裂解液

sufficient for 100 reactions, for molecular biology

同義詞:

Extract-N-Amp 直接PCR血裂解液

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.25
暫時無法取得訂價和供貨情況

等級

Molecular Biology
for molecular biology

形狀

liquid

用途

sufficient for 100 reactions

儲存溫度

−20°C

一般說明

血液裂解溶液可结合血液中和溶液 (N9784)一起使用, 有助于从全血、血卡上干燥的全血和培养的哺乳动物细胞中快速提取和中和 DNA。

该裂解溶液是Extract-N-Amp和REDExtract-N-Amp血液PCR试剂盒的组分

應用

血液裂解液用于在聚合酶链反应 (PCR) 扩增以前裂解血液细胞以提取DNA。[1]它还可用于分离 CRISPR-Cas9 基因编辑的基因组 DNA。 [2][3]

法律資訊

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

象形圖

Corrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
McDermott S M, et al.
Molecular and Cellular Biology, MCB-00790 (2015)
Leandro S Thiago et al.
Haematologica, 99(1), 155-162 (2013-07-23)
The B-cell compartment in which multiple myeloma stem cells reside remains unclear. We investigated the potential presence of mature, surface-membrane immunoglobulin-positive B lymphocytes clonally related to the tumor bone marrow plasma cells among different subsets of peripheral blood B cells
Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
McDermott S M, et al.
Molecular and cellular biology, MCB-00790 (2015)
Aaron Zhong et al.
STAR protocols, 1(2) (2020-10-20)
Reporter cell lines based on human pluripotent stem cells (hPSCs) are highly desirable for studying differentiation, lineage tracing, and target cell selection. However, several technical bottlenecks, such as DNA transduction, low homology recombination rate (HDR), and single-cell cloning, have made
Amber K Bowers et al.
Genetics, 164(4), 1345-1353 (2003-08-22)
To take advantage of available expressed sequence tags and genomic sequence, we have developed 64 PCR-based molecular markers in Chlamydomonas reinhardtii that map to the 17 linkage groups. These markers will allow the rapid association of a candidate gene sequence

Questions

  1. Does L3289 - Lysis Solution for blood come in a size larger than 2.5 mL. I have purchased a 10 mL size in the past but am not seeing that size as an option.

    1 answer
    1. Unfortunately, a 10 mL package size is not available at this time. The 25 mL package size has been discontinued. Should a custom volume quote be of interest, pricing and lead time may be requested at the link below:
      https://www.sigmaaldrich.com/US/en/services/custom-products/bulk-and-custom-services

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