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重要文件

K2638

Sigma-Aldrich

激肽释放酶 来源于人类血浆

buffered aqueous solution, ≥5 units/mg protein

同義詞:

激肽原, 激肽原酶

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54
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形狀

buffered aqueous solution

品質等級

比活性

≥5 units/mg protein

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

human ... KLK1(3816)

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一般說明

Kallikrein肽酶属于15个高度保守的胰蛋白酶或胰凝乳蛋白酶家庭的成员。[1]血浆激肽释放酶(PK)是一种来源于血浆前激肽释放酶的丝氨酸蛋白酶,也是一种在血液循环中含量较高的酶原。[2]KLKB1基因位于人染色体4q35.2上。[3]

應用

从人血浆中提取的激肽释放酶已用于:

  • 培养人肝癌细胞系[4]
  • 研究其对来自脑膜炎奈瑟氏菌的奈瑟球菌肝素结合抗原(NHBA)的裂解作用[5]
  • 肽酶抑制分析[6]

生化/生理作用

血浆激肽释放酶(PK)参与缓激肽的合成,可维持血液代谢物水平和高血压。它还参与凝血因子XII的激活,促进炎症和内源性凝血途径。[2]PK控制心血管系统中的蛋白水解级联反应,如激肽酶激肽系统、肾素血管紧张素系统、纤维蛋白溶解系统和补体旁路等。[7]它参与胰高血糖素样肽-1(GLP-1)和神经肽Y(NPY) 的裂解,这表明血浆激肽释放酶可能会影响代谢和糖尿病。[2]

單位定義

一个酶活性单位是指在pH 8.7、25°C条件下,每分钟将1.0 μMNα-苯甲酰基-L-精氨酸乙酯(BAEE)水解为Nα-苯甲酰基-L-精氨酸和乙醇所需的酶量。

外觀

溶于20 mM Tris-HCl (pH 7.8)和100 mM NaCl的溶液中。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Elisa Pantano et al.
PloS one, 14(8), e0203234-e0203234 (2019-08-02)
Neisserial Heparin Binding Antigen (NHBA) is a surface-exposed lipoprotein of Neisseria meningitidis and a component of the Bexsero vaccine. NHBA is characterized by the presence of a highly conserved Arg-rich region involved in binding to heparin and heparan sulphate proteoglycans
Xueqing Xu et al.
Nucleic acids research, 37(22), 7381-7393 (2009-10-13)
A subtelomeric region, 4q35.2, is implicated in facioscapulohumeral muscular dystrophy (FSHD), a dominant disease thought to involve local pathogenic changes in chromatin. FSHD patients have too few copies of a tandem 3.3-kb repeat (D4Z4) at 4q35.2. No phenotype is associated
Ekaterina Zubareva et al.
Pharmaceuticals (Basel, Switzerland), 14(11) (2021-11-28)
The disfunction or deficiency of the C1 esterase inhibitor (C1INH) is associated with hereditary or acquired angioedema (HAE/AAE), a rare life-threatening condition characterized by swelling in the skin, respiratory and gastrointestinal tracts. The current treatment options may carry the risks
D Kolte et al.
British journal of pharmacology, 162(7), 1639-1649 (2010-12-24)
Kallikrein acts on high molecular weight kininogen (HK) to generate HKa (cleaved HK) and bradykinin (BK). BK exerts its effects by binding to B(2) receptors. The activation of B(2) receptors leads to the formation of tissue plasminogen activator, nitric oxide
Allen P Kaplan et al.
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 104(3), 193-204 (2010-04-10)
To review the mechanisms by which bradykinin is generated in hereditary angioedema (HAE) (C1 inhibitor deficiency), including the role of human plasma proteins and endothelial cells. Published articles in reviewed journals that address (1) the fundamentals of bradykinin formation, (2)

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