K0879

硫酸卡那霉素（来自卡那霉素链霉菌）

powder, γ-irradiated

• 同義詞: 卡那霉素 硫酸酯 来源于卡那霉素链霉菌, 卡那霉素 A, 卡那霉素碱 硫酸盐
• 經驗公式（希爾表示法）: C₁₈H₃₆N₄O₁₁ · H₂O₄S
• CAS號碼: 25389-94-0
• 分子量：: 582.58
• EC號碼: 246-933-9
• MDL號碼: MFCD00070253
• 分類程式碼代碼: 12352200
• PubChem物質ID: 57654313
• NACRES: NA.85

屬性

• 生物源: Streptomyces kanamyceticus
• 等級: Molecular Biology; for molecular biology
• 無菌: γ-irradiated
• 形狀: powder
• 效力: ≥700 μg per mg
• 顏色: white to off-white
• pH值: 6.5-8.5(1% solution)
• 抗生素活性譜: Gram-negative bacteria; Gram-positive bacteria; mycoplasma
• 作用方式: protein synthesis | interferes
• 儲存溫度: 2-8°C
• 適合性: nonselective for Escherichia coli; nonselective for coliforms
• SMILES 字串: OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
• InChI 密鑰: OOYGSFOGFJDDHP-KMCOLRRFSA-N

描述

一般說明

Chemical structure: aminoglycoside

Kanamycin, A from Streptomyces kanamyceticus[1] is a polycationic aminoglycoside[1] and a broad-spectrum antibiotic.[2] Structurally, it comprises hydroxyl and amino functional groups.[1]

應用

Kanamycin sulfate Streptomyces kanamyceticus has been used as an antibiotic component in Murashige and Skoog medium for the selection of tobacco tissue culture explants.[3] It has also been used as an antibiotic in Luria broth for culturing Escherichia coli K-12 strain MG1655 cells for secretion assay[4] and E. coli TV1061 cells for immobilization studies.[5]

生化／生理作用

作用机制：该产品通过与70S核糖体亚基结合，抑制易位并引起错误编码而起作用。

耐药机制：氨基糖苷类修饰酶（包括乙酰转移酶、磷酸转移酶、核苷酸转移酶）可以改变这种抗生素，阻止其与核糖体相互作用。

抗菌谱：硫酸卡那霉素对革兰氏阴性和革兰氏阳性菌以及支原体有效。

Kanamycin′s hydroxyl and amino functional groups are crucial for its membrane action functionality. However, it also exhibits toxicity towards mammalian cells.[1] It prevents contamination in cell culture as well as a selective agent for bacteria with kanamycin resistance.

原則

Kanamycin binds to the 30S ribosomal subunit and inhibits protein synthesis, leading to cell death.

準備報告

直接在小瓶中用无菌水配制储备液 (10mg/mL)。储备液应储存在 2-8°C 下。在 37°C 下可稳定 5 天。

安全資訊

• 象形圖: GHS08
• 訊號詞: Danger
• 危險聲明: H360D
• 防範說明: P201 - P308 + P313
• 危險分類: Repr. 1B
• 儲存類別代碼: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• 水污染物質分類（WGK）: WGK 2
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)