The immunoglobulin concentration of this product is not measured. This is a pooled product from adult AB negative males. Based on information published in the Geigy Scientific Tables, the average immunoglobulin levels for adult males, ages 20 – 70, are as follows:
IgG
10.69 – 12.04 g./L
IgA
1.32 – 2.34 g/L
IgM (not age dependent)
0.77 g/L
暫時無法取得訂價和供貨情況
推薦產品
生物源
human male plasma (Type AB)
品質等級
無菌
sterile-filtered
成份
hemoglobin, ≤30 mg/dL
來源
USA origin
技術
cell culture | mammalian: suitable
cell culture | stem cell: suitable
雜質
≤10 EU/mL endotoxin
運輸包裝
dry ice
儲存溫度
−20°C
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相關類別
應用
人 AB 血清用于组织工程、移植和细胞治疗应用，可用于扩增来自脂肪组织的间充质干细胞 (MSC) 或来自人骨髓的间充质基质细胞； 用于标准化的角膜缘上皮干细胞移植物生成和移植； 用于在造血干细胞扩增培养基中以外周血体外扩增 NK 细胞； 以及移植前人胰岛培养技术的升级。
人血清已被用于：作为含吐温®去污剂的磷酸盐缓冲液 (PBST)的成分用于桥连酶联免疫吸附试验(ELISA); 作为模拟生物样品用于评估复杂生物样品在纸基ELISA试验中的性能; 作为稀释剂用于制备基线样品的稀释系列，从而为添加NISTmAb的血清和多发性骨髓瘤（MM）患者样品MM2232生成标准曲线
生化／生理作用
人AB血清通常是通过全血自然凝结而获取，称为Off-The-Clot (OTC) 血清。由凝结全血获取的人血清是首选的血清材料，因为它通常含有较高浓度的更加多样化的生长因子，可实现最优的细胞生长。来自AB 血型供体的人血清没有A型和B型血抗原的特异性抗体。这一特性令其成为通用的供体血清，适用于各种细胞培养，包括人干细胞系。
包裝
由于产品包装瓶供应不足，不同批次产品的包装可能不同。瓶盖是否包裹热缩膜不会影响产品的完整性/无菌性。
警告
ELISA 检测每位捐献者的乙型&和丙型肝炎无反应，对人类免疫缺陷病毒 (HIV) 抗体无反应。然而，应将人源产品视为潜在传染性产品并进行相应处理。
分析報告
内毒素检测
其他說明
我们的人血清产品呈浑浊状并不罕见。该产品来自混合血清，浑浊表明血清中存在脂质。暂无透明、无脂质的人血清产品。
法律資訊
TWEEN is a registered trademark of Croda International PLC
免責聲明
仅供研究使用。本品在法国的科研应用受到监管，包括用于进口和出口活动（法国《公共卫生法典》Public Health Code Article L 1211-1 paragraph 2）。如《公共卫生法典》所述，购买者（即最终用户）需要从法国研究部(France Ministry of Research)获得进口授权。订购此产品即代表您已确认获得合法的进口授权。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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文章
Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
查找有关FBS和其他血清产品的常见问题解答(FAQ)以及相关产品的链接。
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What is the antibodies concentration in Product H4522, human serum?
1 answer-
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Il prodotto è stato testato per negatività a HSV?
1 answer-
Each donor is tested for and found non-reactive to Hepatitis B & C and non-reactive to Human Immunodeficiency Virus (HIV) antibody by ELISA. HSV is not on the list of agents that are tested. Nevertheless, products of human origin should be considered potentially infectious and handled accordingly. See the link below to review a sample or lot specific Certificate of Analysis for this product: https://www.sigmaaldrich.com/product/sigma/i4506#product-documentation
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If this is "serum" derived from "plasma" the plasma would have contained anti-coagulant. To turn the plasma into serum it has to be clotted. Has this product been clotted to remove the fibrin? Or is it still in fact plasma?
1 answer-
The product has been clotted. Blood is collected and treated with citrate (anticoagulant) which inhibits clotting initially. The blood is then centrifuged. When the spin is complete, layers will appear which allow a differentiation between the cells and the plasma. The plasma is removed and calcium is added to activate the clotting cascade. The preparation is then centrifuged again and the result is a large clot which is pelleted to the bottom of the tube with a serum layer on top.
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Are Human Serum products H4522 and H6914 suitable for complement assays? Is there testing available for complement levels or activity?
1 answer-
Human Serum products H4522 and H6914 may not be suitable for complement assays when stored at -20°C due to potential degradation of complement activity. For complement assays, alternatives like S1764 (Complement sera human) and C9473 (Complement Serum Standard human) are recommended. It is advised to review the Certificates of Analysis (CoAs) for these products to ensure they meet the specific requirements of your complement assay
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Which human serum is recommend for a nucleic acid stability assay, specifically for incubating siRNA in serum to represent bloodstream kinetics?
1 answer-
The use of human serum for a nucleic acid stability assay has not been performed as it is not part of our lab's product characterization focus. However, the citation titled "Improving gene silencing of siRNAs via tricyclo-DNA modification, PMID: 21687522" suggest that product H4522 may be suitable for this application. It is recommended to refer to the publication for additional insights.
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What are the differences between serum and plasma-derived serum?
1 answer-
Whole blood serum is obtained by allowing natural clotting followed by filtration,lacking fibrinogen. Plasma-derived serum is obtained from non-clotted whole blood through centrifugation.
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What Biosafety Level would be suitable for human serum such as H4522, H5667, and H6914?
1 answer-
Human origin products from normal donors should be used at Biosafety Level 2 (BSL-2), which is suitable for handling moderate-risk agents causing human disease. Thus, treat these products as potentially infectious and handle accordingly.
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what does this do
1 answer-
Human AB serum is added as a supplement to culture media at a concentration of 2-10% to provide a mixture of nutritional, hormonal, growth, and attachment factors. The serum also acts as a buffer to the cell culture system against a variety of disruptions to cell growth and toxic effects such as pH change, proteolytic activity, or the presence of heavy metals or endotoxin.
Please see the link below for more information:
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/serum-cell-tissue-faqHelpful?
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Is product H4522 obtained using Institutional Review Board (IRB)-approved consent forms and protocols?
1 answer-
According to the supplier:
Each donor unit used in the preparation of this material was tested and found negative for HBsAG, HBV-NAT, HIV-1/2, HCV, HCV-NAT, HIV-1 NAT, and Syphilis per FDA regulations. The human plasma was collected from male donors' blood type AB in FDA licensed commercial donor establishments located in the USA in accordance with ethical standards. Each donor signed an informed consent form prior to collection, which is traceable by the unique identifiable number to maintain anonymity. A quality assurance system is in place to ensure traceability to individual donor. Documentation to this effect is available upon request.Helpful?
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Could you please confirm if the serum contains normal levels of human complement components?
1 answer-
This product has not been treated to remove or inactivate complement components, however no testing has been performed to confirm these levels. It is a pooled product derived from 50 - 70 donors per lot. Any active complement be expected to meet 'normal levels.
Should complement testing be a requirement, please see product S1764. This is a lyophilized human serum product tested for CH50 activity. See the links below to review a sample Certificate of Analysis and the product datasheet:
S1764 Certificate of Analysis
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/S1764-1MLSLCQ7524.pdfS1764 Datasheet
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/408/363/s1764dat.pdfHelpful?
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