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重要文件

HT5011

Sigma-Aldrich

甲醛溶液,中性缓冲,10%

case of 48 × 15 mL, histological tissue fixative

同義詞:

甲醛

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About This Item

MDL號碼:
分類程式碼代碼:
12352114
PubChem物質ID:
NACRES:
NA.47
暫時無法取得訂價和供貨情況

包裝

case of 48 × 15 mL

濃度

10% formalin (approx. 4% formaldehyde)

顏色

colorless

pH值

6.90-7.10

密度

1.080 g/cm3

應用

hematology
histology

儲存溫度

room temp

SMILES 字串

[O-]#[C+H2]

InChI

1S/CH2O/c1-2/h1H2

InChI 密鑰

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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一般說明

甲醛既是一种非凝固剂又是一种添加剂固定剂。已知甲醛可快速穿透组织,但固定过程可能需要很长时间才能与组织蛋白形成各种交联。福尔马林固定被认为是在醛和蛋白质之间形成交联并产生凝胶,从而将细胞成分维持在其体内的关系。只要经过适当的固定,组织应能承受组织处理或染色的后续阶段。虽然没有真正的通用固定剂,但10%中性缓冲福尔马林(NBF)可能是全世界最常用的光学显微镜固定剂,并也是一种相对宽容的固定剂。在几十种可用的固定剂中,很难找到比10%NBF还更常用的固定剂。10%中性缓冲福尔马林非常适合大通量实验室,并需要相对较短的固定时间,但也可用于组织的长期储存。

應用

10%中性缓冲福尔马林(NBF)是一种常用的通用组织学固定剂。它已用于固定组织、蛋白质复合物和肿瘤样本。[1][2][3]
常用的通用组织学固定剂。

包裝

箱体容器是带有白色防漏盖的聚丙烯,部分预填充了10%NBF。

外觀

中性缓冲的溶液

儲存和穩定性

密封储存在室温。不可冷冻。

相關產品

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

185.0 °F

閃點(°C)

85 °C

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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Questions

1–2 of 2 Questions  
  1. How can a 10% buffered formalin solution be diluted to a 2% solution? Is it necessary to dilute it with buffered solution or can deionized water be used instead?

    1 answer
    1. The HT5011 solutions are advertised as 10% Neutral Buffered Formalin. The formaldehyde content, however, is expected to be in the range of 3.7% to roughly 4.0%. This can be rather confusing because the terms formaldehyde and formalin are often used interchangeably by some. For many histology laboratories, formaldehyde is used to describe formaldehyde content. Some laboratories do consider the terms interchangeable. Formalin is used to describe a dilution from the 37% to 40% stock bottle of the saturated solution. If the 37% to 40% solution is diluted 1 to 10, this is the origin of the term 10% formalin. But the formaldehyde content is still just 3.7% to 4.0%.
      If a simple 2% solution of HT501 is desired, then simply dilute 1 part of HT501 with 4 parts of water. This would result in a formaldehyde content ranging from 0.74% to 0.8%. If you dilute with water, there is a chance the expiration dating could be affected. A better way to dilute 10% formalin would be to create a salt solution used for the original product. To prepare a suitable diluent for dilution, combine 4 grams of sodium phosphate monobasic and 6.5 grams of sodium phosphate, dibasic (anhydrous) in 1 liter of water. The water can be diluted in either distilled or deionized water. If 1 liter of diluent is not required, simply scale down in a proportional amount the amount of chemicals and water used. If the salts are used for dilution, it would probably be best to prepare a 2% solution and use it immediately. If a 2% formaldehyde solution is required, simply dilute the stock HT501 one to one with either water or the salt solution mentioned above. If using water, the same recommendation for immediate use is appropriate.

      Helpful?

  2. Is product HT5011 an IVD product? Is it 100% phosphate buffered?

    1 answer
    1. HT5011 is not a product approved for IVD use. While the product was originally introduced as suitable for IVD use, the IVD status was changed to Research Use Only. The product currently carries a label for Research Use Only and is formulated with formaldehyde, water, and phosphate buffers.

      However, there is a product that is approved for IVD use, which is available in package sizes of 700 mL, 5 L, and 10 L, with the product number 100496.

      Helpful?

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