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Merck
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重要文件

HT10132

Sigma-Aldrich

布安溶液

histological fixative

同義詞:

布安液体

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About This Item

MDL號碼:
分類程式碼代碼:
12171500
NACRES:
NA.47
暫時無法取得訂價和供貨情況

儲存期限

Expiry date on the label

成份

acetic acid, 5%
formaldehyde, 9%
picric acid, 0.9%

顏色

yellow

應用

hematology
histology

儲存溫度

room temp

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一般說明

Bouin ′溶液是一种非凝固性黄色染色固定剂,由饱和苦味酸、甲醛和乙酸组成。

應用

Bouin′溶液是良好的固定剂,可以用来保存柔软和精细的结构。它在各种三色法中作为媒染剂。
此外,它已被用于以下目的:
  • 用于双光子激发荧光(TPEF)和二次谐波发生(SHG)成像的人肝活检组织样品的染色
  • 用于显微镜观察卵泡细胞的大鼠卵巢的固定
  • 用于形态学和组织学分析的螃蟹性腺的固定
用饱和苦味酸、甲醛和乙酸制备。良好的固定剂,可以用来保存柔软和精细的结构。在各种三色法中作为媒染剂。

生化/生理作用

由于其含苦味酸成分,Bouin′溶液用作防腐剂时会使组织收缩。用其固定后,可以保留组织细胞形态和核信息。

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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分析證明 (COA)

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Won-Young Lee et al.
Animal reproduction science, 182, 1-8 (2017-05-04)
Spermatogenesis begins at puberty and continues throughout a male's life. This process is initiated and maintained by spermatogonial stem cells in the seminiferous tubules, and these cells produce haploid spermatozoa. Markers of male germ cells have been fully identified in
Cengiz Yildiz et al.
Cryobiology, 84, 4-9 (2018-09-10)
Grafting of cryopreserved testicular tissue is a promising tool for fertility and testicular function preservation in endangered species, mutant animals, or cancer patients for future use. In this study, we aimed to improve the whole neonatal mouse testicular tissue cryopreservation
Roman Leontovyč et al.
Scientific reports, 9(1), 1347-1347 (2019-02-06)
Trichobilharzia species are parasitic flatworms (called schistosomes or flukes) that cause important diseases in birds and humans, but very little is known about their molecular biology. Here, using a transcriptomics-bioinformatics-based approach, we explored molecular aspects pertaining to the nutritional requirements
Chun-Wei Allen Feng et al.
Scientific reports, 7(1), 17619-17619 (2017-12-17)
Male infertility is a major and growing problem and, in most cases, the specific root cause is unknown. Here we show that the transcription factor SOX30 plays a critical role in mouse spermatogenesis. Sox30-null mice are healthy and females are
Hua Guo et al.
Developmental biology, 430(1), 214-223 (2017-08-16)
GW182 (also known asTNRC6) family members are critically involved in the final effector phase of miRNA-mediated mRNA repression. The three mammalian paralogs, TNRC6a, b and c, are thought to be redundant based on Argonaute (Ago) binding, tethering assays, and RNAi

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