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H8889

Sigma-Aldrich

Histopaque®-1077 Hybri-Max

liquid, sterile-filtered, BioReagent, suitable for hybridoma

同義詞:

Histopaque 1077

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100 ML
500 ML

About This Item

分類程式碼代碼:
12352201
NACRES:
NA.75


履行和交付延遲


Request a Bulk Order

無菌

sterile-filtered

產品線

BioReagent

形狀

liquid

技術

cell culture | hybridoma: suitable
single cell analysis: suitable

雜質

endotoxin, tested

密度

1.077 at 25 °C (relative density)(lit.)

儲存溫度

2-8°C

一般說明

Histopaque®-1077是包含多蔗糖和泛影酸钠的溶液,密度调整到1.077 +/- 0.001 g/cm3[1]这一培养基帮助来自小体积全血的活的淋巴细胞快速恢复。这一技术适合于调节淋巴溶解和人类淋巴细胞抗原(HLA)2分型的细胞。它还可用作T-、B-和“空”淋巴细胞计数前的起始分离步骤。用来建立密度培养基,用于淋巴细胞和其他单核细胞的纯化。

應用

Histopaque®-1077 Hybri-Max已用于分离:
  • 外周血单个核细胞(PBMC)与肝素化血,梯度离心法[2]
  • 来自外周血的免疫细胞(淋巴细胞)[3]
  • PBMC与血液[4]

法律資訊

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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分析證明 (COA)

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Carla Oseroff et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(39), 13980-13985 (2005-09-21)
We have analyzed by ex vivo ELISPOT the anti-vaccinia cytotoxic T lymphocyte responses of peripheral blood mononuclear cells from humans vaccinated with Dryvax vaccine. More than 6,000 peptides from 258 putative vaccinia ORFs predicted to bind the common molecules of
Elliott D SoRelle et al.
eLife, 10 (2021-01-28)
Lymphoblastoid cell lines (LCLs) are generated by transforming primary B cells with Epstein-Barr virus (EBV) and are used extensively as model systems in viral oncology, immunology, and human genetics research. In this study, we characterized single-cell transcriptomic profiles of five
Janet E Alexander et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 73(6), 720-728 (2017-11-11)
As in human populations, advances in nutrition and veterinary care have led to an increase in the lifespan of companion animals. Detrimental physiological changes occurring later in life must be understood before interventions can be made to slow or reduce
Mauricio S Caetano et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 25(24), 7576-7584 (2019-09-22)
Radiotherapy (RT) traditionally has been used for local tumor control in the treatment of cancer. The recent discovery that radiotherapy can have anticancer effects on the immune system has led to recognition of its ability to sensitize the tumor microenvironment
Eiji Yoshihara et al.
Nature, 586(7830), 606-611 (2020-08-21)
Islets derived from stem cells hold promise as a therapy for insulin-dependent diabetes, but there remain challenges towards achieving this goal1-6. Here we generate human islet-like organoids (HILOs) from induced pluripotent stem cells and show that non-canonical WNT4 signalling drives

條款

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

本故障排除指南針對使用 Histopaque 時最常見的錯誤來源進行說明,但並非一份完整的清單。

Questions

1–2 of 2 Questions  
  1. What is the formulation for H8889 Histopaque-1077 Hybri-Max?

    1 answer
    1. The formulation for H8889 Histopaque-1077 Hybri-Max consists of 5.7% polysucrose 400 and 9% sodium diatrizoate.

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  2. Is there a product in your inventory that is comparable to the Corning product 25-072-CV?

    1 answer
    1. Upon comparison of the formulation and product description, the comparable product from MilliporeSigma is identified as product number H8889. Both products, 25-072 and H8889, have a density of 1.077 g/ml and share the same formulation components, including Diatrizoic acid at 96.219 and Polysucrose 400 at 61.363.

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