The use of this product for hydroponic cultures has not been studied, so suitability cannot be confirmed. The 1L container contains 1.6 grams of powder, which is enough to make 1 liter of solution.
推薦產品
相關產品
產品號碼
描述
訂價
訊號詞
Danger
危險聲明
危險分類
Acute Tox. 4 Oral - Eye Dam. 1 - Ox. Sol. 3
儲存類別代碼
5.1B - Oxidizing hazardous materials
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
客戶也查看了
Kristina M Mahan et al.
Journal of industrial microbiology & biotechnology (2018-06-20)
Livestock and fish farming are rapidly growing industries facing the simultaneous pressure of increasing production demands and limited protein required to produce feed. Bacteria that can convert low-value non-food waste streams into singe cell protein (SCP) present an intriguing route
Analysis of arsenic stress-induced differentially expressed proteins in rice leaves by two-dimensional gel electrophoresis coupled with mass spectrometry
Ahsan N, et al.
Chemosphere, 78(3), 224-231 (2010)
Synchronization of cell division in root tips of seven major cereal species for high yields of metaphase chromosomes for flow-cytometric analysis and sorting
Kaeppler H F, et al.
Plant Molecular Biology Reporter, 15(2), 141-147 (1997)
Chromium-induced physiological and proteomic alterations in roots of Miscanthus sinensis
Sharmin S A, et al.
Plant Science, 187, 113-126 (2012)
Jin Seok Yoon et al.
Plant cell reports, 38(9), 1109-1125 (2019-05-28)
BdASR4 expression was up-regulated during abiotic stress and hormone treatments. Plants over-expressing BdASR4 improved drought tolerant. BdASR4 may regulate antioxidant activities and transcript levels of stress-related and abscisic acid-responsive genes. Abiotic stress conditions negatively affect plant growth and developmental processes
文章
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
多年前由 Murashige、Skoog、Gamborg 等先驅開發的經典植物組織培養基，至今仍在植物組織培養研究中扮演著重要的角色。
-
Hello The Hoagland salt is for in vitro cell culture? I need it for hydroponic culture only. you think its suitable? and another question: 1 L means that its the weight of bottle? or it is just enough for making 1 liter soution of Hoagland?
1 answer-
Helpful?
-
-
Good day Do you have Hoagland's solution that supply all elements except Nitrogen?
1 answer-
Currently, this product is the only Hoagland's basal salt mixture available. This is a powdered medium that is prepared at 1.6 grams per liter. The formulation includes Calcium Nitrate. Please see the link below to review the formulation of this and other plant basal salt mixtures and media:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/137/040/h2395for.pdfHelpful?
-
-
Can the Hoagland's solution be autoclaved?
1 answer-
Yes, this product in solution can be autoclaved for sterilization. Please find the preparation instructions below.
This powder is extremely hygroscopic and must be protected from atmospheric moisture. Do not open the container until its contents are allowed to warm to room temperature. If possible the entire contents of the package should be used immediately after opening.
1.6 g of powder is required to prepare 1 L of medium.
Preparing this product in a concentrated form is not recommended as some salt complexes may precipitate. Supplements may be added prior to sterilization or added aseptically to a sterile medium. Certain supplements (i.e. heat labile) may require filter sterilization and may affect the shelf life of the medium.
The basic steps for preparing culture medium are the following:
1. Using a container twice the size of the desired final volume, measure out approximately 90% of the final required volume of tissue culture grade water (e.g. Sigma Product No. W-3500). Example: 900 ml for a final volume of 1000 ml.
2. While stirring, add the powdered product.
3. Rinse the original container with a small volume of tissue culture-grade water to remove traces of the powder. Add to the solution in Step 2.
4. Add any desired supplements (e.g. vitamins, gelling agent, auxin, cytokinin, etc.).
5. While stirring, adjust to the desired pH (e.g. 5.7 +/- 0.1) using KOH, NaOH, or HCl.
6. Add additional tissue culture-grade water to bring the medium to the final volume.
7. If a gelling agent is used, heat the solution to approximately 100°C while stirring.
8. Dispense the medium into culture vessels before or after autoclaving according to your application. Add heat labile constituents after autoclaving.
9. Sterilize the medium in a validated autoclave at 1 kg/cm2 (15 psi). The medium should attain a temperature of 121°C for at least 15 min.All media preparations should be stored at 0-5°C. Store dry powder in a desiccator. Deterioration of powdered medium may be recognized by the following: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) pH change; and 4) inability to promote growth when properly used.
Helpful?
-
-
Why do we use ferric tartarte in this medium. Please specify the role of ferric tartarte. Can ferric tartarte be replaced with ferric sulphate or ferric citrate?
1 answer-
Ferric tartrate is used in the original Hoagland's classical medium formulation developed in the 1930s. This product is a duplication of that formulation. This compound serves as the source of iron in this specific formulation. The most common source of iron utilized in plant basal salt mixtures is Ferrous Sulfate Heptahydrate. Ferric Citrate is less commonly used, however it is present in several Lindemann Orchid salt mixtures. Other iron sources may be suitable, but these have not been evaluated in published classical media formulations.
Helpful?
-
-
Hello, I am in need of some Hoagland solution for my pots. I am in need of 20l of half strength Hoagland solution. Can you provide the technical details and how to use this solution ?
1 answer-
The 1L and 10L package sizes are indicative of the amount of basal salt solution that can be prepared from the powder within. The 1L package contains 1.6 grams and the 10L package contains 16 grams. To prepare at half strength, simply double the volume of water to be added. The 1L package can be diluted in 2L of water and the 10L package can be diluted in 2L of water. Stirring will be required.
Note that this powder is extremely hygroscopic and must be protected from atmospheric moisture. Allow the container to reach room temperature prior to opening.
Please see the link below for additional preparation instructions:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/media-preparationHelpful?
-
-
1.6g of powder makes 1L of solution - how many grams is in the 1L container of powder? I.e. how many litres can be made using the 1L of powder
1 answer-
The 1L and 10L package sizes are indicative of the amount of basal salt solution that can be prepared from the powder within. There is a sufficient amount of powder to prepare either 1 or 10 liters in each bottle, 1.6 grams and 16 grams, respectively. Note that this powder is extremely hygroscopic and must be protected from atmospheric moisture. Allow the container to reach room temperature prior to opening.
Helpful?
-
-
How many L of Hoagland Solution full strength can I prepare with 1.6 g of SKU H2395?
1 answer-
The classical Hoagland's No. 2 formulation shows 1.6 G of the basal salt mixture is sufficient to prepare 1L of medium, This product is a dry powder medium preweighed to prepare the volume stated - 1L and 10L.
Helpful?
-
-
is the hoagland's no. 2 basalt mixture powder 1 ltr, 1 ltr of powder or one ltr of solution or enough powder to make one liter of solution ?
1 answer-
For the SKU H2395-1L, the material is formulated to contain 1.6 grams of powder which is reconstituted to a final volume of 1L of medium.
Helpful?
-
Active Filters
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務
