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重要文件

H1015

Sigma-Aldrich

25-羟基胆甾醇

≥98%

同義詞:

5-胆甾烯-3β,25-二醇

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About This Item

經驗公式(希爾表示法):
C27H46O2
CAS號碼:
分子量::
402.65
Beilstein:
3161259
MDL號碼:
分類程式碼代碼:
41141804
eCl@ss:
39023139
PubChem物質ID:
NACRES:
NA.77
暫時無法取得訂價和供貨情況

生物源

synthetic (organic)

化驗

≥98%

形狀

powder

運輸包裝

ambient

儲存溫度

room temp

SMILES 字串

[H][C@@]12[C@]([C@](CC[C@H](O)C3)(C)C3=CC2)([H])CC[C@@]4(C)[C@@]1([H])CC[C@]4([H])[C@]([H])(C)CCCC(C)(O)C

InChI

1S/C27H46O2/c1-18(7-6-14-25(2,3)29)22-10-11-23-21-9-8-19-17-20(28)12-15-26(19,4)24(21)13-16-27(22,23)5/h8,18,20-24,28-29H,6-7,9-17H2,1-5H3/t18-,20+,21+,22-,23+,24+,26+,27-/m1/s1

InChI 密鑰

INBGSXNNRGWLJU-ZHHJOTBYSA-N

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相關類別

應用

25-羟基胆固醇用于治疗小鼠神经母细胞瘤细胞,以激活 ABCA1 信号[1],并用于与脂肪酸代谢相关的研究。[2][3]

生化/生理作用

25-羟基胆固醇是一种侧链氧甾醇,据报道可引发许多胆固醇分子的活化。这引发了它们从细胞膜到内质网(ER)的运动。[4]25-羟基胆固醇影响免疫系统,并在动脉粥样硬化的发病机制中起关键作用。[5]
抑制甾醇调节元件结合蛋白(SREBPs)的裂解。 25-羟基胆固醇通过下调 Bcl-2 表达和激活半胱天冬酶,从而诱导细胞凋亡。

準備報告

可以在乙醇中制备 25-羟基胆固醇的储备溶液,并将其溶解在细胞培养基中,使最终浓度为1mg/ml。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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存取文件庫

25-hydroxycholesterol enhances cytokine release and toll-like receptor 3 response in airway epithelial cells
Koarai A et al
Respiratory Research, 13, doi: 10-doi: 10 (2012)
Ulf Diczfalusy
Biochimie, 95(3), 455-460 (2012-06-27)
The oxysterol 25-hydroxycholesterol is a widely used compound displaying an array of pharmacological actions in in vitro systems and cell based experimental systems. In spite of the frequent use of this compound over the last few decades and a large number
Alastair G Kerr et al.
The Journal of pharmacology and experimental therapeutics, 361(3), 417-428 (2017-04-01)
Hypercholesterolemia remains one of the leading risk factors for the development of cardiovascular disease. Many large double-blind studies have demonstrated that lowering low-density lipoprotein (LDL) cholesterol using a statin can reduce the risk of having a cardiovascular event by approximately
Marina Parra-Robert et al.
Biomolecules, 9(9) (2019-09-01)
Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic accumulation of lipids. Antisteatotic effects of cerium oxide nanoparticles (CeO2NPs) have recently been shown in animal models of liver disease. However, it is unclear whether the activity of CeO2NPs is related
Brett N Olsen et al.
Biophysical journal, 100(4), 948-956 (2011-02-16)
Side-chain oxysterols are enzymatically generated oxidation products of cholesterol that serve a central role in mediating cholesterol homeostasis. Recent work has shown that side-chain oxysterols, such as 25-hydroxycholesterol (25-HC), alter membrane structure in very different ways from cholesterol, suggesting a

文章

肝脏中胆固醇的合成量由饮食中的胆固醇水平来严格调节。LDL受体调节富含脂质的低密度脂蛋白(LDL)颗粒的细胞转运。

Cholesterol synthesis regulation by dietary levels, LDL receptors control lipid-rich LDL particle transport in cells.

Questions

1–5 of 5 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What is the solubility and solution storage conditions of (2-Hydroxypropyl)-β-cyclodextrin

    1 answer
    1. (2-Hydroxypropyl)-β-cyclodextrin is soluble in water up to 100 mM, and the recommended storage conditions for the prepared solution are at -20°C. It is advisable to use the solution within 1 month once prepared.

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  4. What is the solubility of 25-Hydroxycholesterol, H1015 (CAS number: 2140-46-7), and what are the storage conditions for the prepared solution?

    1 answer
    1. The solubility of 25-Hydroxycholesterol, H1015 (CAS number: 2140-46-7), is up to 20 mM in ethanol. The recommended storage for prepared solutions is at -20°C, and they should be used within 1 month of preparation.

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  5. 안녕하세요. Stock 농도는 어떻게 만들어야할까요?

    1 answer
    1. This product is soluble at 8 mg/mL in ethanol. The stock solution may then be diluted further with culture medium or workiing buffer.

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