推薦產品
包裝
pack of 10 × 1 mL
製造商/商標名
Cytiva 28-9852-55
儲存條件
(20% Ethanol)
床體積
1 mL
容量
~20 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)
儲存溫度
2-8°C
相關類別
一般說明
Protein G GraviTrap™ are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.
應用
特點和優勢
- Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results.
- Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs.
- High purity and recovery with purity >95% in one step and recovery typically 70% to 80%.
- High binding capacity such as Protein G GraviTrap™ ~ 20 mg human IgG/column
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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在實驗室規模下進行脫鹽是一種行之有效、簡單且快速的方法,可快速去除低分子量雜質,同時只需一個步驟即可將樣品轉移到所需的緩衝液中。
條款
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.
相關內容
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare
我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.
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