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GE28-9852-55

Protein G GraviTrap

Cytiva 28-9852-55, pack of 10 × 1 mL

同義詞:

GraviTrap for Protein G, High Performance GraviTrap, Protein G GraviTrap

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

包裝

pack of 10 × 1 mL

製造商/商標名

Cytiva 28-9852-55

儲存條件

(20% Ethanol)

床體積

1 mL

容量

~20 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)

儲存溫度

2-8°C

相關類別

一般說明

Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 mL of Protein G Sepharose 4 Fast Flow.

Protein G GraviTrap are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

應用

Fast and efficient manual purification of monoclonal and polyclonal antibodies

特點和優勢

  • Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs.
  • High purity and recovery with purity >95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as Protein G GraviTrap ~ 20 mg human IgG/column

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


分析證明 (COA)

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Daniel Neumeier et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(18), e2113766119-e2113766119 (2022-04-30)
The capacity of humoral B cell-mediated immunity to effectively respond to and protect against pathogenic infections is largely driven by the presence of a diverse repertoire of polyclonal antibodies in the serum, which are produced by plasma cells (PCs). Recent

文章

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

在實驗室規模下進行脫鹽是一種行之有效、簡單且快速的方法,可快速去除低分子量雜質,同時只需一個步驟即可將樣品轉移到所需的緩衝液中。

條款

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.

相關內容

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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