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GE17-0300-10

Ficoll® PM 400

Cytiva 17-0300-10, pack of 100 g

同義詞:

Density gradient medium

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About This Item

分類程式碼代碼:
12352201
NACRES:
NA.25
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形狀

solid
spray-dried

包裝

pack of 100 g

製造商/商標名

Cytiva 17-0300-10

顏色

White

溶解度

soluble

一般說明

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. The molecules are highly branched and the high content of hydroxyl groups leads to very good solubility in aqueous media.
It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained

特點和優勢

  • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
  • Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
  • Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
  • Better osmotic properties than sucrose.
  • Preserves functional and morphological integrity.
  • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
  • Does not penetrate biological membranes.
  • Serves as the raw material for preparation of Ficoll-Paque gradients.
  • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Ficoll is a registered trademark of Cytiva

儲存類別代碼

13 - Non Combustible Solids


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文章

This page shows methods to remove Percoll from a sample after centrifugation.

Density Marker Beads facilitate Percoll gradient monitoring in biological separations.

密度標記珠有助於生物分離中的 Percoll 梯度監控。

Bøyum 程序所描述的單核細胞分離方法的影響會在下文註明,因為研究情況可能會出現這些影響。

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The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

以下程序已使用 Ficoll-Paque PLUS 進行評估,建議用於分離正常血液樣本,以獲得最大的重複性。

若要製備 Percoll 梯度,必須使用生理鹽水或細胞培養液調整 Percoll 的滲透性,使 Percoll 與生理鹽溶液呈等張性。

相關內容

Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.

編輯資料是為了協助研究人員選擇最可能包含特定細胞或組織類型使用 Percoll 相關資訊的參考資料。

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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