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Merck
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重要文件

G3907

Millipore

谷胱甘肽−琼脂糖

set of 3 pre-packed columns (2.5 ml each), (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

同義詞:

GSH-琼脂糖, S-连接谷胱甘肽琼脂糖

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About This Item

MDL號碼:
分類程式碼代碼:
41106500
NACRES:
NA.56
暫時無法取得訂價和供貨情況

品質等級

形狀

(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

分析物化學類別

proteins (GST)

包裝

set of 3 pre-packed columns (2.5 ml each)

技術

immunoprecipitation (IP): suitable
protein purification: suitable

基質

cross-linked 4% beaded agarose

儲存溫度

2-8°C

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一般說明

这些柱中的树脂由通过硫连接至环氧活化的4%交联琼脂糖珠上的谷胱甘肽组成,形成12个原子间隔基。 [1]

應用

利用谷胱甘肽-琼脂糖的亲和层析技术可以快速、温和、非变性和高度选择性地纯化谷胱甘肽结合酶,如谷胱甘肽-S-转移酶、谷胱甘肽过氧化物酶和乙二醛酶I。[2]
该产品用于新型色胺和四氢-β-咔啉基CDK4选择性抑制剂的设计、合成和生物学评价。[3]它还可用于研究Fascaplysin激发的二吲哚作为CDK4/细胞周期素D1的选择性抑制剂。[4]
利用谷胱甘肽-琼脂糖亲和层析技术,可以快速、温和、非变性和高度选择性地纯化含有谷胱甘肽结合序列的蛋白质,如谷胱甘肽S-转移酶(GST)、谷胱甘肽过氧化物酶和乙二醛酶I。

聯結

产品悬浮液编号G 4510

外觀

0.5 M NaCl + 20%乙醇溶液的混悬液(1:1)

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

180.1 °F

閃點(°C)

82.3 °C


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Paul R Jenkins et al.
Bioorganic & medicinal chemistry, 16(16), 7728-7739 (2008-07-25)
We present the design, synthesis and biological activity of a library of substituted (biphenylcarbonyl)-tryptamine and (biphenylcarbonyl)-tetrahydro-beta-carboline compounds related to the natural product fascaplysin, as novel inhibitors of CDK4/cyclin D1. We show all these molecules, prepared using the Suzuki-Miyaura reaction, being
F Toribio et al.
Journal of chromatography. B, Biomedical applications, 684(1-2), 77-97 (1996-09-20)
The different preparative techniques and related analytical methods used for purification of glutathione peroxidase, glutathione transferase and glutathione reductase, described in papers published in the last ten years, have been reviewed in this article. Among the different purification techniques, chromatography
Dustin L Johnson et al.
Journal of bacteriology, 190(8), 2972-2980 (2008-02-19)
Chlamydophila pneumoniae is a gram-negative obligate intracellular bacterial pathogen that causes pneumonia and bronchitis and may contribute to atherosclerosis. The developmental cycle of C. pneumoniae includes a morphological transition from an infectious extracellular elementary body (EB) to a noninfectious intracellular
Carine Aubry et al.
Organic & biomolecular chemistry, 4(5), 787-801 (2006-02-24)
We present the design, synthesis, and biological activity of three classes of tryptamine derivatives, which are non-planar analogues of the toxic anti-cancer agent fascaplysin. We show these compounds to be selective inhibitors of CDK4 over CDK2, the most active compound
Diane M Kanter et al.
Nucleic acids research, 39(7), 2580-2592 (2010-11-27)
Sld2 is essential for the initiation of DNA replication, but the mechanism underlying its role in replication is not fully understood. The S-phase cyclin dependent kinase (S-CDK) triggers the association of Sld2 with Dpb11, and a phosphomimetic mutation of Sld2

相關內容

利用親和、GST pull-down、TAP 和共免疫沉淀等方法,使用下拉試驗研究體外蛋白質與蛋白質之間的相互作用。

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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