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Merck
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重要文件

G2505

Sigma-Aldrich

N-Glutaryl-L-phenylalanine p-nitroanilide

protease substrate

同義詞:

Glutaryl-L-phenylalanine 4-nitroanilide

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About This Item

經驗公式(希爾表示法):
C20H21N3O6
CAS號碼:
分子量::
399.40
Beilstein:
2919832
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83
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品質等級

化驗

≥98% (HPLC)

形狀

powder

溶解度

methanol with 1 M NH4OH: 50 mg/mL, clear to slightly hazy

儲存溫度

−20°C

SMILES 字串

OC(=O)CCCC(=O)N[C@@H](Cc1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C20H21N3O6/c24-18(7-4-8-19(25)26)22-17(13-14-5-2-1-3-6-14)20(27)21-15-9-11-16(12-10-15)23(28)29/h1-3,5-6,9-12,17H,4,7-8,13H2,(H,21,27)(H,22,24)(H,25,26)/t17-/m0/s1

InChI 密鑰

LFZGBNATHRHOKZ-KRWDZBQOSA-N

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應用

N-Glutaryl-L-phenylalanine p-nitroanilide has been used as a substrate to determine chymotrypsin activity.[1][2]

生化/生理作用

N-Glutaryl-L-phenylalanine p-nitroanilide is useful in testing α-chymotrypsin activity.[3][4]

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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New properties of chymotrypsin modified by fixation with a hydrophobic molecule: hexanal.
M H Remy et al.
Annals of the New York Academy of Sciences, 434, 343-346 (1984-01-01)
S Blais et al.
The Journal of biological chemistry, 268(25), 18637-18639 (1993-09-05)
The Michaelis constant of alpha-chymotrypsin, immobilized on a glutaraldehyde-activated silicate support, for N-glutaryl-L-phenylalanine-p-nitroanilide was determined and was found to be identical with that of the enzyme in solution. The influence of intraparticular diffusion was taken into account by immobilizing different
Daniel Brugger et al.
The British journal of nutrition, 116(3), 425-433 (2016-05-28)
This study investigated the effects of short-term subclinical Zn deficiency on exocrine pancreatic activity and changes in digestive capacity. A total of forty-eight weaned piglets were fed ad libitum a basal diet (maize and soyabean meal) with adequate Zn supply
S V Verevka
Ukrainskii biokhimicheskii zhurnal (1978), 68(3), 36-41 (1996-05-01)
Data on the kinetics of S2'-stimulated alpha-chymotrypsin action have been presented. It is supposed that the increase of the catalytic action of S2'-stimulated chymotrypsin occurs at all three stages of the hydrolytic process-at the enzyme-substrate complex formation, its transformation to
N Spreti et al.
European journal of biochemistry, 268(24), 6491-6497 (2001-12-12)
alpha-Chymotrypsin activity was tested with N-glutaryl-l-phenylalanine p-nitroanilide (GPNA) in aqueous media in the presence of synthetic surfactants, which differ in the flexibility of their bulky head groups. Superactivity can be ascribed to the presence of the tributylammonium residue on the

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