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Merck
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重要文件

G1642

Sigma-Aldrich

sn-Glycerol-3-phosphocholine Phosphodiesterase from mold

lyophilized powder, ≥5 units/mg protein

同義詞:

Glycerophosphorylcholine phosphodiesterase from mold

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54
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形狀

lyophilized powder

品質等級

比活性

≥5 units/mg protein

成份

Protein, ~40% Bradford

儲存溫度

−20°C

單位定義

One unit will produce 1.0 μmole of choline from L-α-glycerophosphorylcholine, G4007, per min at pH 8.0 at 37 °C.

外觀

Lyophilized powder containing Tris buffer salt

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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J Yuan et al.
Neurochemical research, 19(1), 43-48 (1994-01-01)
An enzyme releasing phosphocholine from glycerophosphocholine was purified to apparent homogeneity based upon SDS-PAGE. The enzyme was liberated from lyophilized bovine myelin by differential detergent extraction and final purification was accomplished with Q-Sepharose Fast Flow chromatography yielding an apparently homogeneous
S Sipione et al.
FEBS letters, 384(1), 19-24 (1996-04-08)
In microvessels isolated from bovine brain, microsomal enzyme activities involved in phosphatidylcholine biosynthesis and degradation were determined. The microvessels possessed acyl-CoA:1-acyl-sn-glycero-3-phosphocholine (AT) and glycerophosphocholine phosphodiesterase (GroPChoPDE) activity at a higher level compared with bovine and rat brain or rat liver
J P Galons et al.
Magnetic resonance in medicine, 33(3), 422-426 (1995-03-01)
The purpose of this study was to study the metabolic events during a slow acidosis in three different cell lines by combining 31P magnetic resonance spectroscopy and hollow fiber bioreactor technology. The rate of change in intracellular pH, glycerophosphorylcholine (GPC)
C D Anfuso et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 112(3), 493-501 (1995-11-01)
While steady-state kinetic parameters (metabolite pools, Km and activation energies) are partially known for the enzymes involved in phosphatidylcholine synthesis and degradation in mammalian brain, they are not available for the nervous system of lower vertebrates or invertebrates. Since the
Mária Simocková et al.
The Journal of biological chemistry, 283(25), 17107-17115 (2008-04-25)
The product of the open reading frame YPL206c, Pgc1p, of the yeast Saccharomyces cerevisiae displays homology to bacterial and mammalian glycerophosphodiester phosphodiesterases. Deletion of PGC1 causes an accumulation of the anionic phospholipid, phosphatidylglycerol (PG), especially under conditions of inositol limitation.

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