Please see the link below to review the product technical bulletin. This document contains the preparation, dilution, and working concentrations of the reagents used: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/313/399/flascbul.pdf
推薦產品
儲存溫度
−20°C
相關類別
一般說明
腺苷5′-三磷酸(ATP)生物发光体细胞检测试剂盒用于通过生物发光法测定活性体细胞悬液中释放的腺苷-5′-三磷酸(ATP)。若假定每个活细胞中的ATP含量保持不变，即可推算出细胞浓度。只选择计算活性体细胞数目是因为死亡后细胞中的ATP水平会快速下降。
應用
腺苷5′-三磷酸(ATP)生物发光体细胞检测试剂盒用于准确测定从100多名人类测试患者的血液中分离的嗜中性粒细胞中存在的ATP水平。 [1]
僅套裝組件
產品號碼
描述
- FL-AAB Dilution buffer 1 mL/vial
- FL-AAM Assay mix 1 mL/vial
- FL-AAS ATP standard 1 mL/vial
- FL-SAR Somatic cell releasing reagent 1 mL/vial
訊號詞
Danger
危險聲明
危險分類
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
儲存類別代碼
10 - Combustible liquids
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Is any protocol available for this product? What is the working concentration of each solution? Which reagent should be used to prepare dilutions?
1 answer-
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Can lysates be stored in the Somatic Cell ATP Releasing Reagent of Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC?
1 answer-
In general, no. The working Somatic Cell ATP Releasing Reagent is intended for immediate use, and not for storing ATP in. We have no specific information as to how long ATP will be stable in it.
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What is the optimal dilution for tissue extracts when using Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC?
1 answer-
Sigma-Aldrich has not tested this product with tissue extracts. You will need to perform trials to determine optimal dilution of the sample.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC, be used for adherent cells?
1 answer-
We do not have a separate protocol for adherent cells. However, the somatic cell lysis reagent should be able to lyse cells that are adherent. The reagent contains enough detergent that the cells will be lysed. Once cells are lysed, it is best to assay the sample immediately.
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