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FLASC

Sigma-Aldrich

腺苷 5′-三磷酸 (ATP) 生物发光体细胞检测试剂盒

for cellular ATP determination

同義詞:

ATP bioluminescence assay kit

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160414
NACRES:
NA.26
暫時無法取得訂價和供貨情況

品質等級

200

儲存溫度

−20°C

相關類別

一般說明

腺苷5′-三磷酸(ATP)生物发光体细胞检测试剂盒用于通过生物发光法测定活性体细胞悬液中释放的腺苷-5′-三磷酸(ATP)。若假定每个活细胞中的ATP含量保持不变，即可推算出细胞浓度。只选择计算活性体细胞数目是因为死亡后细胞中的ATP水平会快速下降。

應用

腺苷5′-三磷酸(ATP)生物发光体细胞检测试剂盒用于准确测定从100多名人类测试患者的血液中分离的嗜中性粒细胞中存在的ATP水平。 [1]

僅套裝組件

產品號碼
描述
  • FL-AAB Dilution buffer 1 mL/vial
  • FL-AAM Assay mix 1 mL/vial
  • FL-AAS ATP standard 1 mL/vial
  • FL-SAR Somatic cell releasing reagent 1 mL/vial

象形圖

CorrosionEnvironment
GHS05,GHS09

訊號詞

Danger

危險聲明

H318,H410

危險分類

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

儲存類別代碼

10 - Combustible liquids

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
SLCP4458
SLCJ9482
SLCH0845
SLCC0450
SLCC0453

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Qicheng Ni et al.
Nature communications, 8, 15755-15755 (2017-06-10)
Diabetes is associated with beta cell mass loss and islet dysfunctions. mTORC1 regulates beta cell survival, proliferation and function in physiological and pathological conditions, such as pregnancy and pancreatectomy. Here we show that deletion of Raptor, which is an essential
Lirui Wang et al.
Reproductive toxicology (Elmsford, N.Y.), 75, 23-32 (2017-11-22)
Cadmium (Cd) has been reported to inhibit mouse sperm motility by inducing the tyrosine phosphorylation of dihydrolipoamide dehydrogenase (DLD). This study aimed to assess the potential effects of vitamin C (Vc) on ameliorating Cd-induced tyrosine phosphorylation of DLD and the
Li Xinhong et al.
Theriogenology, 116, 71-82 (2018-05-21)
The reproductive efficiency of Meishan pigs is higher than that of Duroc pigs, but the underlying molecular mechanism for this disparity remains unclear. No systematic quantitative proteomics studies, comparing global proteins in Meishan and Duroc boar spermatozoa have been reported.
Y Zhao et al.
Redox biology, 12, 129-138 (2017-02-24)
The signaling of reactive oxygen species (ROS) is essential for the maintenance of normal cellular function. However, whether and how ROS regulate stem cells are unclear. Here, we demonstrate that, in transgenic mice expressing the human manganese superoxide dismutase (MnSOD)
Linqing Zhen et al.
Reproductive toxicology (Elmsford, N.Y.), 59, 66-79 (2015-11-20)
Hexavalent chromium reportedly induces reproductive toxicity and further inhibits male fertility in mammals. In this study, we investigated the molecular mechanism by which hexavalent chromium affects motility signaling in boar spermatozoa in vitro. The results indicated that Cr(VI) decreased sperm
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Questions

1–5 of 5 Questions  

  1. Is any protocol available for this product? What is the working concentration of each solution? Which reagent should be used to prepare dilutions?

    1 answer

    1. Please see the link below to review the product technical bulletin. This document contains the preparation, dilution, and working concentrations of the reagents used: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/313/399/flascbul.pdf

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  2. Can Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC, be used for adherent cells?

    1 answer

    1. We do not have a separate protocol for adherent cells. However, the somatic cell lysis reagent should be able to lyse cells that are adherent.  The reagent contains enough detergent that the cells will be lysed. Once cells are lysed, it is best to assay the sample immediately.

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  3. Can lysates be stored in the Somatic Cell ATP Releasing Reagent of Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC?

    1 answer

    1. In general, no. The working Somatic Cell ATP Releasing Reagent is intended for immediate use, and not for storing ATP in.  We have no specific information as to how long ATP will be stable in it.

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  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  5. What is the optimal dilution for tissue extracts when using Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC?

    1 answer

    1. Sigma-Aldrich has not tested this product with tissue extracts. You will need to perform trials to determine optimal dilution of the sample.

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