推薦產品
生物源
mouse
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
M1, monoclonal
形狀
buffered aqueous solution
純化經由
using Protein A
物種活性
all
濃度
2-5 mg/mL
技術
western blot: 10 μg/mL
免疫原序列
DYKDDDDK
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
抗 FLAG M1 IgG2b 单克隆抗体与具有游离 N 端的 FLAG 标记物的蛋白结合。
纯化方法-蛋白A
纯化方法-蛋白A
免疫原
FLAG;肽序列 DYKDDDDK
應用
小鼠产生的单克隆抗-FLAG® M1 抗体适用于酵母、动物和大肠杆菌细胞的免疫沉淀、蛋白质印迹或EIA 。
成功使用本产品的应用和相关同行评议文章如下所述。
染色质免疫沉淀(1 篇文章)
免疫荧光(1 篇文章)
请访问我们的FLAG® 应用门户网站,了解更多产品详情。
成功使用本产品的应用和相关同行评议文章如下所述。
染色质免疫沉淀(1 篇文章)
免疫荧光(1 篇文章)
请访问我们的FLAG® 应用门户网站,了解更多产品详情。
生化/生理作用
当 FLAG 表位位于融合蛋白的游离氨基末端时与之结合。不与 Met-FLAG 融合蛋白结合,因此不会识别未处理的、细胞质表达的蛋白。结合具有Ca2+ 依赖性;该复合物在不存在钙离子的情况下解离。
外觀
溶于含 0.02% 叠氮化钠 (w/v) 的 10 mM 磷酸钠、150 mM 氯化钠溶液 (pH 7.4) 中。
準備報告
将抗体稀释至 10ug/mL,置于 0.05 M TBS、pH 7.4、含 1 mM 氯化钙的溶液中。
其他說明
法律資訊
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our 產品選擇工具.
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
客戶也查看了
BMC cancer, 12, 45-45 (2012-01-28)
Spy1 is a novel 'cyclin-like' activator of the G1/S transition capable of enhancing cell proliferation as well as inhibiting apoptosis. Spy1 protein levels are tightly regulated during normal mammary development and forced overexpression in mammary mouse models accelerates mammary tumorigenesis.
Journal of virology, 82(14), 7155-7166 (2008-05-16)
The human immunodeficiency virus type 1 (HIV-1) Tat is a 14-kDa viral protein that acts as a potent transactivator by binding to the transactivation-responsive region, a structured RNA element located at the 5' end of all HIV-1 transcripts. Tat transactivates
Nature communications, 5, 4760-4760 (2014-09-12)
Alternative splicing--the production of multiple messenger RNA isoforms from a single gene--is regulated in part by RNA binding proteins. While the RBPs transformer2 alpha (Tra2α) and Tra2β have both been implicated in the regulation of alternative splicing, their relative contributions
Cell research, 20(2), 211-222 (2010-01-27)
As a critical apoptosis executioner, caspase-3 becomes activated and then enters into the nucleus to exert its function. However, the molecular mechanism of this nuclear entry of active caspase-3 is still unknown. In this study, we revealed that caspase-3 harbors
The Journal of biological chemistry, 284(46), 31726-31734 (2009-09-18)
The regulation of lipid homeostasis by insulin is mediated in part by the enhanced transcription of the gene encoding sterol regulatory element-binding protein-1c (SREBP-1c). The nascent SREBP-1c is embedded in the endoplasmic reticulum (ER) and must be transported to the
我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務