推薦產品
用途
100 assays in 96 well plates
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
组蛋白乙酰转移酶（HAT）在细胞功能中发挥关键作用，它参与基因转录、分化和增殖的调控。HAT活性比色测定试剂盒提供了一种便捷的非放射性系统，可快速、灵敏地检测哺乳动物样品中的HAT活性。该试剂盒包含活性核提取物（NE）阳性对照、HAT辅因子、乙酰辅酶A以及所有下游辅因子和底物。活性HAT催化肽底物的乙酰化，释放出游离形式的辅因子，而辅因子是产生NADH所必需的辅酶。通过与可溶性四唑鎓染料反应，可使用分光光度法轻松检测到NADH。这种检测可以是连续性的，因此适用于动力学研究。该试剂盒为完整测定提供了所有试剂和简单的实验方案。
特點和優勢
- 灵敏可靠的测定
- 利用比色法
- 样品类型：细胞和组织裂解液
- 种属反应性：哺乳动物
- 适用于独立测试或高通量分析和动力学研究
- 简便的过程：将核提取物或蛋白质样品与试剂混合、孵育并读取吸光度
- 方便的96孔微孔板规格
- 持续测量HAT活动；适用于动力学研究
- 无HDAC干扰；可直接使用核粗提物
儲存類別代碼
12 - Non Combustible Liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Sydney P Thomas et al.
eLife, 10 (2021-10-23)
Short-chain fatty acids (SCFAs) acetate, propionate, and butyrate are produced in large quantities by the gut microbiome and contribute to a wide array of physiological processes. While the underlying mechanisms are largely unknown, many effects of SCFAs have been traced
Hiroshi Watanabe et al.
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2091-2096 (2014-01-21)
Aging alters a broad spectrum of physiological, endocrine, and behavioral rhythms, and the close relationship between age-associated disease and disrupted circadian rhythms has been shown. Circadian rhythms are regulated by a set of clock genes and mutations in clock genes
Igor Efimov et al.
FEBS letters, 588(5), 701-704 (2014-01-21)
We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em=-115 mV ± 2 mV, pH 7.0)
Koji Maemura
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2124-2129 (2014-01-21)
Cardiovascular function exhibits circadian oscillation. Furthermore, the onset time and severity of cardiovascular diseases exhibit diurnal variation. The internal circadian clock whose center exists in the suprachiasmatic nucleus regulates this circadian variation. Many studies have reported that night shift workers
Michiko Konno
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2171-2178 (2014-01-21)
Mood disorders show a common feature of distorted cycling of biological systems, manifesting, for example, as diurnal mood variation in depression, phasic time course in bipolar disorder, and seasonal mood swing in seasonal affective disorder. Accordingly, circadian dysfunction has been
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務