推薦產品
籤條
3X FLAG tagged
等級
for molecular biology
形狀
buffered aqueous solution
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
p3XFLAG-CMV™-14 表达载体是分子量6.3 kb 的pCMV51衍生物，用于哺乳动物细胞内 C-末端 3XFLAG™融合蛋白的瞬时或稳定胞内表达。该载体编码多克隆区下游三个相邻的 FLAG®表位 (Asp-Tyr-Lys-Xaa-Xaa-Asp) 。这在采用ANTI-FLAG M2抗体时会导致检测灵敏度上升。当使用SV40 T抗原表达宿主时，可以实现理想的复制效率和基因组整合。
p3XFLAG-CMV-7-BAP 对照质粒是分子量6.2 kb的pCMV5衍生物，用于N末端3XFLAG细菌碱性磷酸酶融合蛋白在哺乳动物细胞中的瞬时细胞内表达。该载体编码多克隆区上游三个相邻的 FLAG 表位 (Asp-Tyr-Lys-Xaa-Xaa-Asp) 。这在采用ANTI-FLAG M2抗体时会导致检测灵敏度上升。第三FLAG 表位包括肠激酶识别序列，可以使3XFLAG肽从纯化的融合蛋白上裂解。
载体图谱和序列
p3XFLAG-CMV-7-BAP 对照质粒是分子量6.2 kb的pCMV5衍生物，用于N末端3XFLAG细菌碱性磷酸酶融合蛋白在哺乳动物细胞中的瞬时细胞内表达。该载体编码多克隆区上游三个相邻的 FLAG 表位 (Asp-Tyr-Lys-Xaa-Xaa-Asp) 。这在采用ANTI-FLAG M2抗体时会导致检测灵敏度上升。第三FLAG 表位包括肠激酶识别序列，可以使3XFLAG肽从纯化的融合蛋白上裂解。
载体图谱和序列
成分
- p3XFLAG-CMV™-14 表达载体 20 μg（E4901），浓度为 0.5mg/ml，在含有 1mM EDTA 的 10mM Tris-HCl（pH8.0）中提供。
- p3XFLAG-CMV™-7-BAP 对照质粒 20 μg（C7472），浓度为 0.5mg/ml，在含有 1mM EDTA 的 10mM Tris-HCl（pH8.0）中提供。
原則
人巨细胞病毒的启动子调节区驱动 FLAG®-融合构建体的转录
其他說明
浏览我们有关其他应用的参考文献，请访问 FLAG® 文献页面。
法律資訊
该产品受 Sigma-Aldrich 公司所拥有的以下专利保护：US6,379,903、US7,094,548、JP4405125、EP1220933、CA2386471 和 AU774216。
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
10 - Combustible liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Viorel Simion et al.
JCI insight, 5(21) (2020-10-07)
Long noncoding RNAs (lncRNAs) play important roles in regulating diverse cellular processes in the vessel wall, including atherosclerosis. RNA-Seq profiling of intimal lesions revealed a lncRNA, VINAS (Vascular INflammation and Atherosclerosis lncRNA Sequence), that is enriched in the aortic intima
Irina Malyukova et al.
Investigative ophthalmology & visual science, 47(1), 206-212 (2005-12-31)
The present study compared properties of wild-type and mutated mouse and human myocilin (Myoc) proteins as a prerequisite for development of a mouse model of glaucoma. cDNA encoding full-length mouse Myoc was cloned into the p3XFLAG-CMV-14 vector. Tyr423His and Ile463Ser
James A Snipes et al.
The Journal of pharmacology and experimental therapeutics, 313(2), 668-676 (2005-01-15)
A splice variant of cyclooxygenase-1 (COX-1), COX-1b (previously termed as COX-3), has been identified in canine tissues as an acetaminophen-sensitive isoform, but the sequence of COX-1b mRNA and the encoded protein are not known in rats. We cloned and sequenced
Yan Qun Chen et al.
The Journal of biological chemistry, 283(15), 10048-10057 (2008-02-02)
AGPAT6 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family that appears to be important in triglyceride biosynthesis in several tissues, but the precise biochemical function of the enzyme is unknown. In the current study, we show that AGPAT6 is
Bengt Phung et al.
PloS one, 6(8), e24064-e24064 (2011-09-03)
The development of melanocytes is regulated by the tyrosine kinase receptor c-KIT and the basic-helix-loop-helix-leucine zipper transcription factor Mitf. These essential melanocyte survival regulators are also well known oncogenic factors in malignant melanoma. Despite their importance, not much is known
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