推薦產品
等級
for molecular biology
形狀
buffered aqueous glycerol solution
分子量
28 kDa
UniProt登錄號
儲存溫度
−20°C
基因資訊
Escherichia coli K12 ... xthA(946254)
一般說明
一种可催化从dsDNA的3′-末端进行单核苷酸去除的3′→5′核酸外切酶。该酶还具有嘌呤和嘧啶内切核酸酶、3′-DNA磷酸酶和RNase H活性。活性高度依赖于温度、盐浓度以及酶与DNA的比例，因此反应条件必须针对特定应用进行优化。
應用
适用于：
- 生产链特异性探针
- 用于Sanger双脱氧测序的单链模板的制备
- 定点诱变
成分
核酸外切酶III是以溶于5 mM 磷酸钾（pH 6.5）、200 mM KCl、0.05 mM EDTA、5 mM 2- 巯基乙醇、200 μg/ml BSA和50%甘油的溶液形式提供的。
單位定義
在37 °C下，一个单位可在30分钟内可从经超声处理的小牛胸腺DNA中释放1 nmole的酸可溶性核苷酸。
相關產品
產品號碼
描述
訂價
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Jan Silhan et al.
Nucleic acids research, 40(5), 2065-2075 (2011-11-10)
We have previously demonstrated that the two Exonuclease III (Xth) family members present within the obligate human pathogen Neisseria meningitidis, NApe and NExo, are important for survival under conditions of oxidative stress. Of these, only NApe possesses AP endonuclease activity
Hui Chen et al.
Chemical communications (Cambridge, England), 48(2), 269-271 (2011-11-23)
We describe herein a novel exonuclease III aided amplification method based on single walled carbon nanotube quenching (EASQ) for sensitive and convenient nucleic acid detection, which enabled 80-fold decrease of detection limit for HIV1 DNA assay compared with no target
Lu Peng et al.
Biosensors & bioelectronics, 35(1), 475-478 (2012-04-03)
We have developed a novel DNA assay based on exonuclease III (ExoIII)-induced target recycling and the fluorescence quenching ability of graphene oxide (GO). This assay consists of a linear DNA probe labeled with a fluorophore in the middle. Introduction of
Xiaolei Zuo et al.
Chembiochem : a European journal of chemical biology, 12(18), 2745-2747 (2011-11-30)
We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon. We demonstrate the specific detection of as few as 30 telomerase-positive breast cancer cells in
Xu-Hua Zhao et al.
Analytica chimica acta, 727, 67-70 (2012-05-01)
Based on the super fluorescence quenching efficiency of graphene oxide and exonuclease III aided signal amplification, we develop a facile, sensitive, rapid and cost-effective method for DNA detection. In the presence of target DNA, the target-probe hybridization forms a double-stranded
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