D6319
Anti-DCP2 (C-terminal) antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
同義詞:
Anti-DCP2 decapping enzyme homolog (S. cerevisiae), Anti-NUDT20, Anti-Nucleleoside diphosphate-linked moiety X motif 20, Anti-Nudix motif 20
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About This Item
分類程式碼代碼:
12352203
NACRES:
NA.41
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生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~50 kDa
物種活性
rat, mouse, human
濃度
~1 mg/mL
技術
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 celle
indirect immunofluorescence: suitable
western blot: 2-4 μg/mL using lysaes of K-562 and Rat1 cells
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... DCP2(167227)
mouse ... Dcp2(20640)
rat ... Dcp2(291604)
一般說明
Dcp2 colocalizes with Dcp1 in distinct cytoplasmic foci along with other proteins involved in the 5′ to 3′ mRNA decay. These foci are termed PB (processing bodies) or DCP-bodies. hDCP2 contains a highly conserved Nudix (nucleoside diphosphate linked to an X moiety) motif critical for the decapping activity.
免疫原
synthetic peptide corresponding to amino acids 406-420 of human DCP2, conjugated to KLH. The corresponding sequence is identical in rat and mouse.
應用
Anti-DCP2 (C-terminal) antibody has been used in immunocytochemistry and image correlation analysis.[1] It may also be used in immunoblotting.
Anti-DCP2 antibody produced in rabbit is suitable for indirect immunofluorescence at a working concentration of 2-5μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human DCP2 and western blot analysis at a working concentration of 2-4μg/mL using lysates of K-562 and Rat1 cells. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
生化／生理作用
Dcp2 is an RNA binding protein and can cleave only a cap structure that is linked to an RNA moiety, suggesting that Dcp2 can differentially associate with specific mRNAs. Dcp2 cleaves the m7G mRNA cap in the 5′ to 3′ mRNA decay pathway, in association with Dcp1 and Hedls complex. Decapping is a critical and highly regulated step in the turnover of mRNA which involves decapping enzymes that hydrolyze the cap structure at the 5′ mRNA. Dcp2 is the catalytic subunit, and the mRNA is degraded by the major cytoplasmic 5′ to 3′ exonuclease XRN1. The enzymatic activity of DCP2 is critically dependent on the DCP1 subunit in vivo.
標靶描述
DCP2 (C-terminal) is a key component of an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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分析證明 (COA)
Lot/Batch Number
Bidirectional regulation of P body formation mediated by eIF4F complex formation in sensory neurons.
Ohannes K Melemedjian et al.
Neuroscience letters, 563, 169-174 (2013-10-02)
Processing (P) bodies are RNA granules that comprise key cellular sites for the metabolism of mRNAs. In certain cells, including neurons, these RNA granules may also play an important role in storage of mRNAs in a translationally dormant state. Utilizing
Lee Davidson et al.
The EMBO journal, 31(11), 2566-2578 (2012-04-24)
Eukaryotic protein-coding genes are transcribed as pre-mRNAs that are matured by capping, splicing and cleavage and polyadenylation. Although human pre-mRNAs can be long and complex, containing multiple introns and many alternative processing sites, they are usually processed co-transcriptionally. Mistakes during
T Dunckley et al.
The EMBO journal, 18(19), 5411-5422 (1999-10-03)
The major pathway of mRNA degradation in yeast occurs through deadenylation, decapping and subsequent 5' to 3' exonucleolytic decay of the transcript body. To identify proteins that control the activity of the decapping enzyme, which is encoded by the DCP1
You Li et al.
Molecular and cellular biology, 28(3), 939-948 (2007-11-28)
mRNA decapping is a critical step in the control of mRNA stability and gene expression and is carried out by the Dcp2 decapping enzyme. Dcp2 is an RNA binding protein that must bind RNA in order to recognize the cap
Christy Fillman et al.
Current opinion in cell biology, 17(3), 326-331 (2005-05-20)
Decapping is a central step in eukaryotic mRNA turnover. Recent studies have identified several factors involved in catalysis and regulation of decapping. These include the following: an mRNA decapping complex containing the proteins Dcp1 and Dcp2; a nucleolar decapping enzyme
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