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D4812

Sigma-Aldrich

REDAccuTaq® LA DNA Polymerase

High fidelity Taq with inert dye, 10X buffer included

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About This Item

MDL號碼:
MFCD01092095
分類程式碼代碼:
12352202
NACRES:
NA.55

形狀

liquid

用途

sufficient for 250 reactions

特點

Long & Accurate PCR
dNTPs included: no
hotstart: no

濃度

1 unit/μL

技術

PCR: suitable

顏色

red

輸入

purified DNA

運輸包裝

wet ice

儲存溫度

−20°C

相關類別

一般說明

REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods.
REDAccuTaq® long and accurate (LA) DNA Polymerase is a blend of Taq DNA polymerase and a small amount of thermostable proofreading polymerase that exhibits 3′→5′ exonuclease and an inert red dye that acts as a tracer and loading buffer. It is suitable for amplifying complex DNA templates like human genomic DNA.

應用

REDAccuTaq® LA DNA Polymerase produce long DNA amplicons for:
  • Mutation analysis
  • Cloning genes
  • DNA sequencing
  • cDNA library generation
  • Amplicon sequencing

特點和優勢

  • Increased fidelity, up to 6.5× that of Taq DNA polymerase
  • Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA
  • Visual confirmation that enzyme has been added, and proper mixing has occurred
  • No additional loading dyes needed. A post-reaction aliquot can be directly used on an agarose gel for electrophoresis

包裝

The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates.

單位定義

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

其他說明

Store REDAccuTaq LA DNA Polymerase and AccuTaq LA 10x Buffer at –20 °C.

法律資訊

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
REDAccuTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Sarah L Batt et al.
Journal of clinical microbiology, 43(6), 2656-2661 (2005-06-16)
Serotyping Streptococcus pneumoniae is a technique generally confined to reference laboratories, as purchasing pneumococcal antisera is a huge investment. Many attempts have been made to modify serological agglutination techniques to make them more accessible, and more recently developments in serotyping
Claudia Knief et al.
Applied and environmental microbiology, 71(7), 3826-3831 (2005-07-08)
Three upland soils from Thailand, a natural forest, a 16-year-old reforested site, and an agricultural field, were studied with regard to methane uptake and the community composition of methanotrophic bacteria (MB). The methane uptake rates were similar to rates described
Microbial investigations in Opalinus Clay, an argillaceous formation under evaluation as a potential host rock for a radioactive waste repository
Poulain S, et al.
Geomicrobiology Journal, 240-249 (2008)
Matthew J Ellis et al.
Breast cancer research and treatment, 119(2), 379-390 (2009-10-22)
Mutations in the alpha catalytic subunit of phosphoinositol-3-kinase (PIK3CA) occur in approximately 30% of ER positive breast cancers. We therefore sought to determine the impact of PIK3CA mutation on response to neoadjuvant endocrine therapy. Exons 9 (helical domain) and 20
Maren Godmann et al.
Mechanisms of development, 126(8-9), 650-664 (2009-06-23)
Krüppel-like factor 4 (Klf4, GKLF) was originally characterized as a zinc finger transcription factor essential for terminal differentiation and cell lineage allocation of several cell types in the mouse. Mice lacking Klf4 die postnatally within hours due to impaired skin
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End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

相關內容

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

Long and Accurate PCR Amplification of DNA with RedAccuTaq® (D4812)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA

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