CWA-1158

Anti-Human CD276/B7-H3 (376.96) ColorWheel^® Dye-Ready mAb

• 同義詞: CD276 antigen;4Ig-B7-H3;B7 homolog 3;B7-H3;Costimulatory molecule
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 376.96, monoclonal
• 產品線: ColorWheel^®
• 形狀: lyophilized
• 分子量: calculated mol wt 57.24 kDa
• 純化經由: using protein G
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 技術: flow cytometry: suitable
• 同型: IgG2aκ
• Protein ID登錄號: NP_079516.1
• UniProt登錄號: Q5ZPR3
• 相容性: for use with ColorWheel^® Dyes (Required, (Sold Separately))
• 環保替代類別: , Aligned
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CD276(80381)

描述

一般說明

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

特異性

Clone 376.96 is a mouse monoclonal antibody that detects CD276/B7-H3. It targets an epitope within the extracellular domain.

免疫原

Cultured COLO 38 human melanoma cells.

應用

Quality Control Testing

Evaluated by Flow Cytometry in SK-OV-3 cells.

Flow Cytometry Analysis: Staining of one million SK-OV-3 cells was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1158, Anti-Human CD276/B7-H3 (376.96) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1016 Mouse IgG2a isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million SK-OV-3 cells was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1158, Anti-Human CD276/B7-H3 (376.96) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or a 1:1 mixture of an equivalent amount of Cat. No. CWA-1016 Mouse IgG2a isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

相容性

Requires ColorWheel^® Dye (Sold Separately)

標靶描述

CD276 antigen (UniProt: Q5ZPR3; also known as 4Ig-B7-H3, B7 homolog 3, B7-H3, Costimulatory molecule, CD276) is encoded by the CD276 (also known as B7H3, PSEC0249, UNQ309/PRO352) gene (Gene ID: 80381) in human. CD276 is a single-pass type I membrane protein that is member of B7 immunoregulatory family. It is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to produce the mature form, which contains an extracellular domain (aa 29-466), a short transmembrane domain (aa 467-487), and a cytoplasmic domain (aa 488-534). It participates in the regulation of T-cell-mediated immune response. It is a ubiquitous protein but is undetectable in peripheral blood lymphocytes or granulocytes and is only weakly expressed in resting monocytes. It is also expressed in dendritic cells derived from monocytes and in epithelial cells of sinonasal tissue, extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. It plays a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Higher expression of CD276 has been shown in a variety of cancer types, including breast, pancreatic, and ovarian cancer and its expression has been correlated with poor survival in ovarian cancer subjects. It is suggested to play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis. Its levels are also reported to be up-regulated in cells that mediate rejection of human transplants. This ColorWheel^® Dye-Ready monoclonal antibody was developed using our Cat. No. MABC1731, Anti-B7-H3 (CD276) Antibody, clone 376.96. (Ref.: Fauci, JM., et al. (2014). Gynecologic Oncology 132(1); 203-210; Chen, Y-W., et al. (2008). Curr. Can. Drug Targets. 8(5); 404-413).

外觀

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重構

1.0 mg/mL after reconstitution at 25 µL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

法律資訊

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable