CMC0024

CONTROLLER BL21(DE3)化学感受态细胞

Escherichia coli, rod shaped

• 分類程式碼代碼: 12352200
• NACRES: NA.85

屬性

• 產品名稱: CONTROLLER BL21(DE3)化学感受态细胞, for controlled protein expression
• 生物源: Escherichia coli
• 等級: Molecular Biology; for molecular biology
• 增長模式: adherent or suspension
• 形態學: rod shaped
• 技術: microbiological culture: suitable
• 細胞轉化: competent cell type: chemically competent; transformation efficiency: >1 x 10⁷ cfu/μg
• 運輸包裝: dry ice
• 儲存溫度: −70°C

描述

一般說明

The CONTROLLER BL21(DE3) Chemically Competent Cells are an E. coli strain optimized for high efficiency transformation.
They are ideal for cloning and propagation of plasmid clones. They give high yield and high quality plasmid DNA.
Genotype

F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)

特點和優勢

• Reduce leaky expression: The LacI repressor protein binds to the lacO operator to block transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter will be greatly reduced.
• High protein yields: Both 10G and BL21(DE3)-based strains are noted for their ability to express high levels of protein. For expression from a T7 promoter please select the HI-Control BL21(DE3) strain. This strain also is deficient in proteases, yielding more stable protein preparations. Most other promoters can be used in either HI-Control strain.
• Terrific efficiency: The simple, reliable heat-shock procedure for chemical transformation gives excellent results from small amounts of plasmid DNA.

成分

• CONTROLLER BL21(DE3) chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for expression

安全資訊

• 儲存類別代碼: 10 - Combustible liquids