LB83

98062328, human blood, Lymphoblastoid

• 分類程式碼代碼: 41106514

屬性

• product name: LB83, 98062328
• 生物源: human blood
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblastoid
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human lymphocytes, Xeroderma pigmentosum, EBV-transformed

細胞系描述

Established by EBV transformation of mononuclear cells from the peripheral blood of a 15 year old female. The patient has been diagnosed with Xeroderma pigmentosum (XP) complementation group G, showed no skin cancer or neurologic abnormalities and reduced NK activity. XP is a relatively rare recessively inherited disease with DNA repair deficiency. Fibroblasts from the same individual have been designated XP125LO.

應用

DNA repair defect studies, hprt mutation analysis

培養基

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

例行更新培養

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml 5%

其他說明

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