The Jurkat E6.1 cell line is a banked product of ECACC and is tested sterility, viability, mycoplama, and identity. It is not tested for any specific expression. The depositor listed in Prof H Harris/Dr C Madin, Sir William Dunn School of Pathology, Oxford. The line has not been further modified. Various publications suggest that the E6.1 does express CD28, however we cannot validate these claims. It may be helpful to refer to the literature for further information.
Jurkat E6.1 Cell Line human
88042803, human blood (leukemic T-cell lymphoblast), Not specified
同義詞:
Jurkat Cells, Jurkat Clone E6-1 Cells
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About This Item
分類程式碼代碼:
41106514
生物源:
human blood (leukemic T-cell lymphoblast)
增長模式:
Suspension
染色體組型:
Pseudodiploid, modal No 46
形態學:
Not specified
產品:
Interleukin 2 (IL-2), human alpha interferon
受體:
Not specified
相關疾病:
cancer
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產品名稱
Jurkat E6.1 Cell Line human, 88042803, from human blood(leukemic T-cell lymphoblast)
生物源
human blood (leukemic T-cell lymphoblast)
增長模式
Suspension
染色體組型
Pseudodiploid, modal No 46
形態學
Not specified
產品
Interleukin 2 (IL-2), human alpha interferon
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human leukaemic T cell lymphoblast
細胞系描述
Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.
應用
IL-2 assay and cloning studies
Jurkat E6.1 cell line has been used to:
- study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate[1]
- determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components[2]
- study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro[3]
DNA分析
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18
培養基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.
其他說明
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文章
CellASIC® ONIX2 微流控系統如何用於分析 caspase-3 介導的細胞凋亡/細胞死亡及活體免疫和癌症細胞系的細胞缺氧的應用說明。
Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.
條款
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
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Does this particular batch of Jurkat E6.1 express CD28?
1 answer-
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I am interested in Jurkat cells; product number 88042803 appears to be a vial of these cells for culturing. But why is the extension '-DNA-5UG' in the product number? Does this mean that it is not cells but 5 µg DNA isolated from Jurkat cells???
1 answer-
To order the actual cells, order the SKU with the -1VL suffix, 88042803-1VL. This SKU is for the Jurkat E6.1 cells that can be propagated and maintained in culture. The other SKUs listed there are preparations of DNA, RNA and cDNA from this cell line.
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