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BF-11

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

分類程式碼代碼:
41106514
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生物源

Rat x Mouse Hybridoma

包裝

tube of 5 μg 95050413-DNA-5UG
pkg of vial of cells 95050413-1VL

ImmunoDon

Rat AC1 spleen cells

骨髓瘤

Sp2/0 Ag-14

反應

Anti-Erythropoietin, human

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Rat ACI spleen x Mouse Sp2/0 Ag-14

細胞系描述

The BF-11 hybridoma cell line secretes a rat monoclonal antibody named Epoclonalan that reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The α, β and asialo forms of human erythropoietin are bound by the antibody to the same extent.

免疫原

Human erythropoietin

應用

Immunological studies, isolation of erythropoietin by immuno-affinity methods.
The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

培養基

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO2; 37°C.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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