MABF1096

Anti-LRBA Antibody, clone 2D4.1

clone 2D4.1, from mouse

• 同義詞: Lipopolysaccharide-responsive and beige-like anchor protein, Beige-like protein, CDC4-like protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2D4.1, monoclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μg
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_006717
• UniProt登錄號: P50851
• 目標翻譯後修改: unmodified
• 基因資訊: human ... LRBA(987)

描述

一般說明

Lipopolysaccharide-responsive and beige-like anchor protein (UniProt: P50851; also known as Beige-like protein, CDC4-like protein) is encoded by the LRBA (also known as BGL, CDC4L, LBA) gene (Gene ID: 987) in human. LRBA is a single-pass membrane protein that displays ubiquitous expression in normal tissues, but its expression is elevated in malignancy. In immune cells. It is found in all membrane compartments that are associated with receptor signaling, including the plasma membrane, Golgi complex, clathrin-coated pits, and trans-endocytic vesicles. Its expression is induced in B cells and macrophages by bacterial lipopolysaccharides (LPS). Two isoforms of LRBA have been reported that are produced by alternative splicing. LRBA contains 2 BEACH (Beige and Chediak-Higashi) domains and six WD repeats at its C-terminal. LRBA may be involved in coupling signal transduction and vesicle trafficking to enable polarized secretion and/or membrane deposition of immune effector molecules. It coordinates signaling of immune receptors to promote effector function and plays a crucial role in immune regulation. Mutations in LRBA gene are associated with defective B-cell differentiation and decreased or absent antibody production. (Ref.: Park, MY et al. (2016). Nat. Scientific Reports 6:36568).

特異性

Clone 2D4.1 detects human Lipopolysaccharide-responsive and beige-like anchor protein (LRBA). It targets an epitope within 115 amino acids from the N-terminal half.

免疫原

GST/His-tagged recombinant fragment corresponding to 115 amino acids from the N-terminal half of human Lipopolysaccharide-responsive and beige-like anchor protein (LRBA).

應用

Anti-LRBA, clone 2D4.1 Antibody, Cat. No. MABF1096 is a mouse monoclonal antibody that detects Lipopolysaccharide-responsive and beige-like anchor protein (LRBA) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected LRBA in human pancreas, human testis, and human cervical cancer tissue sections.

Research Category
Inflammation & Immunology

品質

Evaluated by Western Blotting in Jurkat cell membrane preparations.

Western Blotting Analysis: 1 µg/mL of this antibody detected LRBA in 10 µg of Jurkat cell membrane preparations.

標靶描述

~320 kDa observed; 319.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable