PI.1 Ut

90111909, raccoon uterus, Fibroblast-like

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: PI.1 Ut, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 90111909
• 生物源: raccoon uterus
• 包裝: tube of 5 μg 90111909-DNA-5UG; pkg of vial of cells 90111909-1VL
• 增長模式: Adherent
• 染色體組型: 2n = 38
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Racoon uterus

細胞系描述

Derived from trypsinised uterine tissue of an adult female Racoon. Pl.1Ut was developed for studies of feline and canine viruses since the Racoon is one of the few animals which is susceptible to both.

應用

Virus studies: feline and canine viruses

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm² by washing 3 times with 0.255 trypsin or trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan; maximum 90 passages expected. Growth rate is very slow.

其他說明

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