A-72

89050908, dog thigh, Fibroblast-like

• 分類程式碼代碼: 41106514

屬性

• product name: A-72, 89050908
• 生物源: dog thigh
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Canine Golden Retriever tumour

細胞系描述

Derived from tumour of thigh (unknown histology) of 8 year old Golden Retriever which had a previous history of mammary adenocarcinoma. Susceptible to canine corona-, canine Ad type I and II, canine herpes, canine parainfluenza and canine parvo- viruses.

應用

Virus studies: Canine corona-, Ad I and II, parainfluenza, herpes and parvo- viruses

培養基

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 0.02% EDTA; 37°C. CO₂ is detrimental; free gas exchange with atmospheric air necessary.

其他說明

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