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重要文件

COS-1 Cell Line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

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About This Item

分類程式碼代碼:
41106514
暫時無法取得訂價和供貨情況

生物源

African green monkey kidney

包裝

tube of 5 μg 88031701-DNA-5UG
pkg of vial of cells 88031701-1VL

增長模式

Adherent

染色體組型

Not specified

形態學

Fibroblast

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

相關類別

細胞系來源

Monkey African green kidney, SV40 transformed

細胞系描述

Derived from CV-1 simian cells transformed by origin-defective mutant of SV40 which codes for wildtype T Ag. Possible host for propagation of pure populations of recombinant SV40 virus. The cells should be handled under laboratory containment level 2.

應用

COS-1 cell line has been used to test the toxicity of various concentrations of iron oxide nanoparticles (for use in medical diagnostics).[1] It has also been used to study the interaction of nanoparticles with their cellular targets.[2]
Virus replication studies: SV40

培養基

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

例行更新培養

Split semi-confluent cultures 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. In order to prevent syncitia formation, do not allow cells become confluent.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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Lot/Batch Number

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Questions

1–7 of 7 Questions  

  1. Are my Cell Line cells mycoplasma tested?

    1 answer

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

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  2. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 answer

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

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  3. What medium should I use when I thaw the Cell Line cells?

    1 answer

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

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  4. What passage are my Cell Line cells?

    1 answer

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

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  5. Are the Cell Line cells tested for viral pathogens?

    1 answer

    1. HPA Cultures does not perform any viral testing on the cell lines.

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  6. How do I handle Cell Line frozen cells upon arrival?

    1 answer

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

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  7. How many Cell Line cells are in the vial?

    1 answer

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

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