Spontaneous contraction, such as beating, of HL-1 cells may not be observable by bright-field microscopic inspection due to its subtlety. The beating phenotype can be observed using a fluorescent calcium flux dye, Fluo-8®.
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產品名稱
HL-1 心肌细胞系, HL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels.
生物源
mouse
品質等級
技術
cell based assay: suitable
cell culture | mammalian: suitable
一般說明
HL-1心肌细胞系是永生化的小鼠心肌细胞系，能够连续分裂并自发收缩，同时保持分化的心脏表型。 HL-1可以连续传代，而不会失去其分化的心肌细胞表型，包括形态、生化和电生理特性。HL-1细胞已用于研究正常心肌细胞在信号传导、电、代谢和转录调控方面的功能，以及解决诸如缺氧、高血糖-高胰岛素血症、细胞凋亡和缺血再灌注等病理状况。
HL-1系源自从成年雌性C57BL/6J小鼠切除的AT-1皮下肿瘤。亲本AT-1系最初源自在转基因小鼠中生长的心房肿瘤，其中SV40大T抗原的表达通过心房利钠因子（ANF）启动子靶向心房心肌细胞。
HL-1系源自从成年雌性C57BL/6J小鼠切除的AT-1皮下肿瘤。亲本AT-1系最初源自在转基因小鼠中生长的心房肿瘤，其中SV40大T抗原的表达通过心房利钠因子（ANF）启动子靶向心房心肌细胞。
細胞系描述
心肌细胞
品質
• 每瓶含有≥ 1X106 个活细胞。
•Charles River动物诊断服务通过小鼠必要透明面板PCR面板对细胞进行传染病检测，结果为阴性。
•细胞对支原体污染呈阴性。
•功能测试：基于Fluo-8钙染料的荧光检测细胞内钙的变化
•Charles River动物诊断服务通过小鼠必要透明面板PCR面板对细胞进行传染病检测，结果为阴性。
•细胞对支原体污染呈阴性。
•功能测试：基于Fluo-8钙染料的荧光检测细胞内钙的变化
儲存和穩定性
HL-1心肌细胞系应保存在液氮中。在初始解冻后，细胞可以培养至少10代，而不会显著影响细胞标志物的表达和功能。
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本产品含有转基因生物（GMO）。在欧盟范围内，转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。这项法律规定{HCompany}有义务要求您提供某些信息，以及有关处理 GMO 的机构的信息。请点击这里，获得最终用户声明（EUD）表格。
本产品含有转基因生物（GMO）。在欧盟范围内，转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。这项法律规定{HCompany}有义务要求您提供某些信息，以及有关处理 GMO 的机构的信息。请点击这里，获得最终用户声明（EUD）表格。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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相關內容
Learn how to use our cell culture tested, ready-to-use MilliShot™ single dose antibiotic solutions, conveniently packaged in one-time use vials.
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Hello, Are these HL1 cells the type that do not beat in culture? Please answer, yes or no.
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Hello, Are these HL1 cells the type that do not beat in culture?
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HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation as well as to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion.
The HL-1 line was derived from AT-1 subcutaneous tumor excised from an adult female C57BL/6J mouse. The parental AT-1 line was originally derived from an atrial tumor growing in a transgenic mouse in which expression of the SV40 large T-antigen was targeted to atrial cardiomyocytes via the atrial natriuretic factor (ANF) promoter.
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