EIII

86072804, Human x Mouse Hybridoma hybrid, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• product name: EIII, 86072804
• 生物源: Human x Mouse Hybridoma hybrid
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: pJ3.11, p79a, CFTR DNA markers
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human x mouse hybrid

細胞系描述

This cell line may contain a sub-chromosomal fragment of human chromosome 17 bearing markers thought at the time to flank the cytotic fibrosis gene. The human DNA was donated from the human osteosarcoma cell line MNNG-HOS.

應用

Somatic cell hybrid mapping

培養基

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

其他說明

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