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Sigma-Aldrich

磷酸钙转染试剂盒

Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

同義詞:

Gene delivery

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About This Item

EC號碼:
233-140-8
分類程式碼代碼:
12352200
NACRES:
NA.85

等級

for molecular biology

品質等級

200

形狀

solution

用途

 kit sufficient for 160 transfections (6 cm dishes)
 kit sufficient for 400 transfections (3.5 cm dishes)
 kit sufficient for 80 transfections (10 cm dishes)

技術

transfection: suitable

運輸包裝

dry ice

儲存溫度

−20°C

相關類別

一般說明

磷酸钙法转染是将DNA引入真核细胞的常用方法。这一技术已经在广泛的细胞类型中使用，可实现瞬时和稳定的转染效果。

應用

磷酸钙转染试剂盒用于：
  • 启动转染
  • 转染 Hek293T 细胞
  • 转染 H29D 细胞

适用于瞬时和稳定地将DNA转染至培养的哺乳动物细胞中。 使用磷酸钙方法已成功转染了以下细胞：

BAEC
肠黑素瘤细胞
CHO K1
COS-7
成纤维细胞（人胚胎、新皮肤）
HEK293
Huh7
IMR-90
LLC（Lewis肺癌）
NIH3T3
PC-12
PCI-13
SH-SY5Y
SK-HEP-1
T47D

特點和優勢

  • 适用于瞬时和稳定转染
  • 可重复用于多种细胞类型
  • 广泛引用
  • 价格便宜

成分

磷酸钙转染试剂盒包含：
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES 缓冲盐 (H1012)
25 ml 分子生物学级纯水(W4502)

原則

该方法是基于将含有磷酸钠的HEPES缓冲盐水和含有DNA的CaCl2 溶液进行缓慢混合实现的。 所形成的DNA-磷酸钙共沉淀物可粘附于细胞表面，并可能通过胞吞作用被细胞吸收。

其他說明

该方案可用于使用pSV40-CAT质粒作为报告基因对CHO细胞进行瞬时转染。

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H319

危險分類

Eye Irrit. 2

儲存類別代碼

12 - Non Combustible Liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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M Wigler et al.
Cell, 14(3), 725-731 (1978-07-01)
Previous studies from our laboratories have demonstrated the feasibility of transferring the thymidine kinase (tk) gene from restriction endonuclease-generated fragments of herpes simplex virus (HSV) DNA to cultured mammalian cells. In this study, high molecular weight DNA from cells containing
Munjin Kwon et al.
Methods in molecular biology (Clifton, N.J.), 1018, 107-110 (2013-05-18)
The calcium phosphate transfection is a widely used method for introducing foreign DNA plasmids into cells. Mechanisms underlying this transfection method are not yet defined; however, DNA-calcium phosphate precipitates are internalized by the cells and DNA is efficiently expressed in
A new technique for the assay of infectivity of human adenovirus 5 DNA.
F L Graham et al.
Virology, 52(2), 456-467 (1973-04-01)
Denise A Carbonaro et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 22(3), 607-622 (2013-11-22)
Gene transfer into autologous hematopoietic stem cells by γ-retroviral vectors (gRV) is an effective treatment for adenosine deaminase (ADA)-deficient severe combined immunodeficiency (SCID). However, current gRV have significant potential for insertional mutagenesis as reported in clinical trials for other primary
Paolo Conrotto et al.
Molecular oncology, 5(6), 527-537 (2011-09-02)
The transcription factor SOX11 is a novel diagnostic marker for mantle cell lymphoma (MCL), distinguishing this aggressive tumor from potential simulators. Recent data also show that the level of SOX11 correlates to in vitro growth properties in MCL, as well
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文章

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

細胞轉染簡介

轉染將遺傳物質引入細胞，有助於基因表達和細胞生物學的研究。

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

條款

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

磷酸鈣轉染試劑組規程

使用磷酸鈣將 DNA 植入真核細胞是一種常用的轉染方法。此轉染方案可針對多種細胞類型進行最佳化。

相關內容

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

轉染試劑選擇指南

瀏覽我們便捷的轉染試劑選擇指南，為您的特定細胞系和應用需求匹配最佳試劑。

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

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