推薦產品
形狀
lyophilized powder
比活性
100-300 units/mg protein
分子量
~175 kDa
成份
Protein, 65-85%
異物活動
Creatinase and urease ≤1%
Hexokinase and ATPase ≤0.1%
儲存溫度
2-8°C
生化／生理作用
来自假单胞菌属的肌酐酶是一种每个亚基分子量为28.4 kDa的同六聚体酶。它是一种环状酰胺水解酶，可催化肌酸酐向肌酸的可逆性转化。每个单体在β-链的C末端和主要α-螺旋的N末端附近都含有一个双核锌中心。这些锌离子指示着活性位点的位置。[2]
物理性質
等电点： 4.7
米氏常数：3.2 x 10‾2M（肌苷），5.7 x 10‾2M（肌酸）
结构：每摩尔的酶6个亚基（每个亚基结合一摩尔的锌）
抑制剂：Ag+、Hg++、N-溴代琥珀酰亚胺、EDTA
最适pH值： 6.5 − 7.5
最适温度： 70°C
pH稳定性： pH 7.5 − 9.0 (5°C，16小时)
热稳定性： 低于70°C（pH 7.5，30分钟）
米氏常数：3.2 x 10‾2M（肌苷），5.7 x 10‾2M（肌酸）
结构：每摩尔的酶6个亚基（每个亚基结合一摩尔的锌）
抑制剂：Ag+、Hg++、N-溴代琥珀酰亚胺、EDTA
最适pH值： 6.5 − 7.5
最适温度： 70°C
pH稳定性： pH 7.5 − 9.0 (5°C，16小时)
热稳定性： 低于70°C（pH 7.5，30分钟）
單位定義
在pH 8.0、25 °C条件下，一个单位将在每分钟内水解1.0 μmole的肌苷
外觀
含有蔗糖和BSA作为稳定剂的冻干粉末
分析報告
通过缩二脲法测定的蛋白质。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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條款
Protocol for the Assay Procedure for Creatininase
相關內容
Protocol for the Assay Procedure for Creatininase
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