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B2025

Sigma-Aldrich

亮蓝 G-胶体浓缩液

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.85

形狀

solution

儲存溫度

room temp

一般說明

考马斯亮蓝染色剂在十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS PAGE) 中被广泛用于蛋白质的检测。亮蓝 G 染色因其灵敏度接近银染法而被称为蓝银染色。考马斯亮蓝 G 染色液通过疏水作用与蛋白质结合。它可以有效染色角膜前囊膜,毒性相对较小。

應用

亮蓝 G-胶体浓缩液用于在分析酿酒酵母中的
  • 转录因子(TFIID)复合物前,进行体外胃蛋白酶消化后卵巢肿瘤液蛋白质组中
    • 的蛋白质片段的染色

  • 重構

    用 800 mL 水稀释至 1 L 后,混悬液将含有 0.1% (w/v) 亮蓝 G、0.29 M 磷酸和 16% 饱和硫酸铵。

    分析報告

    已在 SDS-PAGE 上检测了本品的适用性。

    相關產品

    產品號碼
    描述
    訂價

    象形圖

    Corrosion

    訊號詞

    Warning

    危險聲明

    危險分類

    Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

    儲存類別代碼

    8B - Non-combustible corrosive hazardous materials

    水污染物質分類(WGK)

    WGK 2

    閃點(°F)

    Not applicable

    閃點(°C)

    Not applicable


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    Purification of Active TFIID from Saccharomyces cerevisiae EXTENSIVE PROMOTER CONTACTS AND CO-ACTIVATOR FUNCTION
    Auty R, et al.
    The Journal of biological chemistry, 279(48), 49973-49981 (2004)
    Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels
    Dyballa N and Metzger S
    Journal of Visualized Experiments (2009)
    Julie Ducreux et al.
    Bioconjugate chemistry, 19(10), 2088-2094 (2008-09-24)
    PEGylation of antibodies is known to increase their half-life in systemic circulation, but nothing is known regarding whether PEGylation can improve the inhibitory potency of antibodies against target receptors. In this paper, we have examined this question using antibodies directed
    A multi-laboratory evaluation of a common in vitro pepsin digestion assay protocol used in assessing the safety of novel proteins
    Thomas K, et al.
    Regulatory Toxicology and Pharmacology, 39(2), 87-98 (2004)
    Antonis Ververis et al.
    Cellular and molecular life sciences : CMLS, 73(1), 163-184 (2015-07-15)
    Katanins are microtubule (MT)-severing AAA proteins with high phylogenetic conservation throughout the eukaryotes. They have been functionally implicated in processes requiring MT remodeling, such as spindle assembly in mitosis and meiosis, assembly/disassembly of flagella and cilia and neuronal morphogenesis. Here

    條款

    Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.

    相關內容

    Brilliant Blue G-Colloidal Concentrate has been designed for post-electrophoresis staining of proteins in IEF, PAGE, and SDS-PAGE gels. Fixing the proteins prior to staining is recommended for maximum sensitivity.

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