推薦產品
品質等級
效力
>5000 mg/kg LD50, oral (Rat)
技術
protein staining: suitable
pH值
6.4 (20 °C, 10 g/L in H2O)
溶解度
40 g/L
體積密度
520 kg/m3
儲存溫度
no temp limit
InChI
1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1
InChI 密鑰
CMGWKYXJIYYESN-UHFFFAOYSA-M
相關類別
一般說明
Coomassie亮蓝G 250 (C.I.42655)是Coomassie亮蓝(CBB)之一，是一种三苯基甲烷染料。CBB染料广泛用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的蛋白质显色。CBB G-250（绿蓝色）染料适用于Bradford测定的蛋白定量。
應用
Coomassie亮蓝G 250 (C.I.42655)已用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中的凝胶染色。它还用于Bradford蛋白测定。
分析報告
鉴定标准（UV/VIS-光谱）：通过测试
最大吸光值λ最大（缓冲液pH7.0）：577 - 584 nm
光谱吸光性A 1%/1cm（λ最大；0.01 g/l；缓冲液pH 7.0；基于干物质计算）：450 - 570
TLC-测试：通过测试
干燥损失（100 °C）： ≤ 8 %
电泳适用性：通过测试
最大吸光值λ最大（缓冲液pH7.0）：577 - 584 nm
光谱吸光性A 1%/1cm（λ最大；0.01 g/l；缓冲液pH 7.0；基于干物质计算）：450 - 570
TLC-测试：通过测试
干燥损失（100 °C）： ≤ 8 %
电泳适用性：通过测试
法律資訊
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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Robert G E Krause et al.
Analytical biochemistry, 566, 107-115 (2018-11-21)
Coomassie brilliant blue R250, an anionic dye is the most popular stain to detect proteins resolved in SDS-PAGE gels. Crystal violet, a cationic dye was found to be versatile and stained proteins in SDS-PAGE gels and in zymograms. Stained proteins
Nazim Mekaoui et al.
Journal of chromatography. A, 1232, 134-141 (2011-12-22)
Commercial samples of Coomassie Brilliant Blue G-250 (CBB) were not pure enough to give reliable results when used as indicator of amine content in biological material. The polar and apolar impurities produce unacceptable biases in the results. Counter current chromatography
Gang Lu et al.
Bio-protocol, 10(6), e3565-e3565 (2021-03-05)
Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purification have been published. Here, we report a protocol for large-scale purification of Rice stripe virus (RSV) from RSV-infected rice
Mohammad-Bagher Ebrahim-Habibi et al.
Scientific reports, 9(1), 1273-1273 (2019-02-06)
Investigation of non-covalent interaction of hydrophobic surfaces with the protein G (PrG) is necessary due to their frequent utilization in immunosensors and ELISA. It has been confirmed that surfaces, including carbonous-nanostructures (CNS) could orient proteins for a better activation. Herein
文章
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
找出 SDS-PAGE 樣品製備挑戰的原因和補救方法，並優化電泳條件。
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