推薦產品
用途
sufficient for 60 cell suspensions
包裝
pkg of 1 kit
技術
flow cytometry: suitable
應用
cell analysis
detection
檢測方法
fluorometric
運輸包裝
wet ice
儲存溫度
2-8°C
相關類別
應用
特點和優勢
与使用生物素或地高辛偶联的 dUTP 或使用荧光染料（荧光素或 BODIPY）结合的脱氧核苷酸相比，Br-dUTP 的结合量更大，有助改善检测性能。
包裝
该试剂盒分两部分保存于湿冰中运输。收货后，APO-PART1 在 −20 °C、APO-PART2在2-4 °C下储存。
原則
与用较大染料（如 FITC、生物素或地高辛）标记的脱氧尿苷三磷酸相比，Br-dUTP 更容易结合到DNA 片段中。判定细胞凋亡的生物学特征之一是基因组 DNA 降解为 180-200 bp 的片段，通常称为“DNA 断裂“（DNA laddering）。 片段化使得 DNA 断裂处产生大量 3′-羟基位点。 这一特性被用于APO-BRDU 试剂盒，通过用溴脱氧尿苷三磷酸（Br-dUTP）标记 3′-羟基位点来识别凋亡细胞。 通过末端转移酶 (TdT) Br-dUTP 酶促连接双链或单链 DNA 的 3-羟基位点。 由于缺乏可用的 3-羟基位点，非凋亡细胞不结合 Br-dUTP。
僅套裝組件
產品號碼
描述
- Br-dUTP 480 μL
- Fluorescein PRB-1 antibody 300 μL
- Negative control cells 5 mL
- PI/RNase staining buffer 30 mL
- Positive control cells 5 mL
- Reaction buffer .6 mL
- Rinsing buffer 120 mL
- Terminal deoxynucleotidyl transferase (TdT) 45 μL
- Wash buffer 120 mL
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訊號詞
Danger
危險分類
Carc. 1B - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
3 - Flammable liquids
閃點（°F）
55.4 °F
閃點（°C）
13 °C
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