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重要文件

A7420

Sigma-Aldrich

淀粉葡萄糖苷酶 来源于黑曲霉

greener alternative

lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose

同義詞:

1,4-α-D-葡聚糖葡萄糖水解酶, 糖化淀粉酶, 葡萄糖淀粉酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54
暫時無法取得訂價和供貨情況

形狀

lyophilized powder

品質等級

比活性

30-60 units/mg protein (biuret)

成份

protein, ≥80%

環保替代產品特色

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

雜質

≤0.02% glucose

環保替代類別

儲存溫度

−20°C

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一般說明

我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。该产品针对淀粉乙醇研究进行了优化,可提高能效并防止浪费。有关详细信息,请参阅 《Biofiles》的文章

應用

来自黑曲霉的淀粉葡萄糖苷酶用于水解α-D-葡糖苷。 它可用于啤酒的酿造以及面包和果汁的生产。 Sigma的淀粉葡萄糖苷酶被用于将糖原水解成葡萄糖单体,以研究骨骼肌中的脂质积累[1]

生化/生理作用

来自黑曲霉的淀粉葡萄糖苷酶能够水解低聚糖的α-D-(1-4)、α-D-(1-6)和α-D-(1-3)糖苷键。[2]淀粉葡萄糖苷酶是一种胞外酶,可将淀粉转化成糊精和葡萄糖。该酶在淀粉加工工业中用于由玉米糖浆商业生产D-葡萄糖。[3][4]

單位定義

在pH 4.5、55℃条件下,一单位酶在3 min内可从淀粉中释放出1.0 mg葡萄糖。

外觀

含有低于0.02%葡萄糖的冻干粉

其他說明

抑制劑

產品號碼
描述
訂價

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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Youngmi Kim et al.
Bioresource technology, 99(12), 5165-5176 (2007-11-09)
DDGS and wet distillers' grains are the major co-products of the dry grind ethanol facilities. As they are mainly used as animal feed, a typical compositional analysis of the DDGS and wet distillers' grains mainly focuses on defining the feedstock's
Yi-Chen Lin et al.
PloS one, 12(5), e0177115-e0177115 (2017-05-05)
It has been proposed that malto-oligosaccharides (MOSs) are possibly recycled back into amylopectin biosynthesis via the sequential reactions catalyzed by plastidial α-glucan phosphorylase 1 (Pho1) and disproportionating enzyme 1 (Dpe1). In the present study, the reciprocal co-immunoprecipitation experiments using specific
Alisa K Manning et al.
Frontiers in genetics, 11, 615-615 (2020-08-06)
Causal transcripts at genomic loci associated with type 2 diabetes (T2D) are mostly unknown. The chr8p23.1 variant rs4841132, associated with an insulin-resistant diabetes risk phenotype, lies in the second exon of a long non-coding RNA (lncRNA) gene, LOC157273, located 175
Erick Perera et al.
Scientific reports, 10(1), 18676-18676 (2020-10-31)
The aim of this work was to evaluate two functional feeds for the gilthead seabream, Sparus aurata, containing low inclusion of two microalgae-based products (LB-GREENboost, LBGb; and LB-GUThealth, LBGh). Fish (12-13 g) were fed for 13 weeks a control diet or one
Jaetaek Kim et al.
Molecular endocrinology (Baltimore, Md.), 22(11), 2531-2543 (2008-09-20)
The receptors for IGF-I (IGF-IR) and insulin (IR) have been implicated in physiological cardiac growth, but it is unknown whether IGF-IR or IR signaling are critically required. We generated mice with cardiomyocyte-specific knockout of IGF-IR (CIGF1RKO) and compared them with

條款

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

本程序可用於以澱粉為底物的淀粉葡萄糖苷酶活性的測定。

Questions

1–5 of 5 Questions  
  1. What is the shelf life of a solution (1 mg/mL in water) of Product A7420, Amyloglucosidase from Aspergillus niger and how should it be stored?

    1 answer
    1. The stability of this product in solution has not been evaluated. However, 172426 Glucoamylase (syn. Amyloglucosides) from Rhizopus, notes that solutions are stable when stored frozen in aliquots at -20°C for up to 3 months. Similar stability would be expected for A7420. Avoid freeze/thaw cycles of solutions.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. What is the molecular weight of Product A7420,  Amyloglucosidase from Aspergillus niger?

    1 answer
    1. Amyloglucosidase from A. niger exists in two isoforms with MWs of 69,810 and 89,130, as determined by SDS-PAGE. See: Starch, 34, 346 (1982).

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  4. Does Product A7420,  Amyloglucosidase from Aspergillus niger, contain any stabilizers or additives?

    1 answer
    1. The starting material for Product No. A7420 is Product No. A3042, which is a preparation of the enzyme in a 1 M glucose solution. This is dialyzed against water until the glucose concentration is less than 0.02%, and then lyophilized.

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  5. How can a solution of Product A7420,  Amyloglucosidase from Aspergillus niger, be prepared?

    1 answer
    1. A 1 mg/mL solution may be prepared in water.

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