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A3151

Sigma-Aldrich

亲和素-过氧化物酶

lyophilized powder

同義詞:

亲和素-POD

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

avidin from egg white
enzyme from horseradish

品質等級

共軛

peroxidase conjugate

形狀

lyophilized powder

成份

Protein, ≥70% E1%/280

標籤範圍

0.7-2.0 mol peroxidase per mol avidin

技術

direct ELISA: 1:50,000

溶解度

H2O: soluble 1 mg/mL
PBS: soluble

儲存溫度

−20°C

一般說明

亲和素是来自于蛋清的同源四聚体蛋白(68kDa)。这种蛋蛋白质与生物素高度结合。因此,亲和素­生物素结合已被用于免疫检测,以检测组织中的抗原定位。亲和素-生物素免疫检测的使用增强了该技术的灵敏度并改善了对低含量抗原的检测。

應用

为了检测小鼠血清中的Ga1缺陷型IgA1抗体,可在96孔板中使用血清进行ELISA检测,其中,血清与生物素化HAA(结合IgA1)一起孵育。然后将HRP偶联的亲和素与血清在37℃孵育40分钟。
亲和素-过氧化物酶已被用于检测抗5-溴脱氧尿苷(抗BrdU)结合和OVA-IgG1

外觀

含有柠檬酸盐缓冲液的冻干粉末。

準備報告

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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P Birner et al.
The American journal of pathology, 158(6), 1991-1996 (2001-06-08)
Monoclonal antibody MIB-1 is a reliable tool for determining proliferating cells in human tissues, but does not react with the homologous mouse antigen and is therefore useless in experimental pathology using mice as model systems. Standard method for assessment of
Luigia Pace et al.
Journal of immunology (Baltimore, Md. : 1950), 184(11), 5969-5979 (2010-04-30)
Type I IFNs are central to a vast array of immunological functions. Their early induction in innate immune responses provides one of the most important priming mechanisms for the subsequent establishment of adaptive immunity. The outcome is either promotion or
S de la Barrera et al.
Clinical and experimental immunology, 138(1), 128-138 (2004-09-18)
Activation of T cells requires both TCR-specific ligation and costimulation through accessory molecules during T cell priming. IFNgamma is a key cytokine responsible for macrophage activation during Mycobacterium tuberculosis (Mtb) infection while IL-10 is associated with suppression of cell mediated
Peter Patrikios et al.
Brain : a journal of neurology, 129(Pt 12), 3165-3172 (2006-08-22)
Although spontaneous remyelination does occur in multiple sclerosis lesions, its extent within the global population with this disease is presently unknown. We have systematically analysed the incidence and distribution of completely remyelinated lesions (so-called shadow plaques) or partially remyelinated lesions
Gautam N Shenoy et al.
Clinical & translational immunology, 10(2), e1246-e1246 (2021-02-09)
With a rapidly growing list of candidate immune-based cancer therapeutics, there is a critical need to generate highly reliable animal models to preclinically evaluate the efficacy of emerging immune-based therapies, facilitating successful clinical translation. Our aim was to design and

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