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Merck
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文件

A3054

Sigma-Aldrich

琼脂糖

For pulsed field electrophoresis sample preparation

同義詞:

3,6-Anhydro-α-L-galacto-β-D-galactan

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About This Item

CAS號碼:
9012-36-6
EC號碼:
232-731-8
MDL號碼:
MFCD00081294
分類程式碼代碼:
12352201
PubChem物質ID:
24890482
NACRES:
NA.21

生物源

algae (red)

形狀

powder

技術

electrophoresis: suitable

雜質

≤10% water

EEO

≤0.10

轉變溫度

gel point ≤30 °C

負離子痕跡

sulfate (SO42-): ≤0.15%

異物活動

DNase and RNase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI 密鑰

MJQHZNBUODTQTK-WKGBVCLCSA-N

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應用

专用于制备染色体 DNA 样品，然后进行脉冲场凝胶电泳 (PFGE)。形成凝胶块用于分离高分子量的 DNA，可以避免传统分离会遇到的剪切。包埋在琼脂糖块中时，细胞可溶解且由限制性内切酶消化 DNA。

分析報告

以下是与我们的琼脂糖相关的性质列表：
硫酸盐含量 - 用作纯度指标，因为硫酸盐是其中存在的主要离子基团。
凝胶强度 - 破裂凝胶所需施加的力。
胶凝点 - 琼脂糖水溶液在冷却时的胶凝温度。 琼脂糖溶液在液体-凝胶转变的过程中会表现出滞后现象 - 也就是说，它们的凝胶点与它们的熔点温度不同。
电内渗（EEO）- 液体通过凝胶的一种运动。琼脂糖凝胶中的阴离子基团被固定在基质上不能移动，但可解离的抗衡阳离子可以向基质中的阴极迁移，从而产生EEO。 由于生物聚合物的电泳运动通常是朝向阳极的，因此EEO会因为内部的对流而破坏分离。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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