This product is a conjugate of 5'-ATP to crosslinked 4% beaded agarose (activated by cyanogen bromide), via the C-8 atom of 5'-ATP. A 9-atom matrix spacer arm connects the ATP and the agarose. Please see the link below to review the product datasheet for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/174/887/a2767pis.pdf
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生物源
plant (Sea weed)
形狀
lyophilized powder
標籤範圍
1-5 μmol per mL
基質
cross-linked 4% beaded agarose
基質活化
cyanogen bromide
基質結合
C-8
基質墊片
9 atoms
儲存溫度
−20°C
SMILES 字串
[X]Nc1ncnc2[n](cnc21)[C@@H]3O[C@@H]([C@H]([C@H]3O)O)CO[P](=O)(O[P](=O)(O[P](=O)(O)O)O)O
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相關類別
一般說明
5′-三磷酸腺苷-琼脂糖（5′-ATP-琼脂糖）是5-ATP通过5′-ATP的C-8原子与交联4%珠状琼脂糖形成的缀合物（由溴化氰激活）。5′-ATP–琼脂糖适用于各种蛋白质和酶的亲和纯化，如周期蛋白依赖性激酶2（CDK2）、热休克蛋白和隐花色素。
應用
5′-三磷酸腺苷-琼脂糖（5′-ATP–琼脂糖）适用于各种蛋白质和酶的亲和纯化，如周期蛋白依赖性激酶2（CDK2）、热休克蛋白-70（HSP-70）和隐花色素。5′-ATP琼脂糖已用于亲和色谱中，以从艾氏腹水癌肿瘤细胞中纯化尿苷激酶。
腺苷5′-三磷酸琼脂糖（5′-ATP琼脂糖）已用于亲和色谱中，以从艾氏腹水癌肿瘤细胞中纯化尿苷激酶。
外觀
用乳糖稳定化的冻干粉
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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Where is the linker attached on the ATP molecule
1 answer-
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How can I hydrate Product A2767, Adenosine 5´-triphosphate-Agarose?
1 answer-
The resin can be hydrated by placing it in excess water, approximately 50 mL per g of resin, for at least 30 minutes. Remove the lactose stabilizer by washing the resin on a Buchner funnel with gentle vacuum, using approximately 100 mL of water per g of resin. Do not allow the resin to dry. Resuspend the resin in excess water or starting buffer to pack the column bed.
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How can I regenerate Product A2767, Adenosine 5´-triphosphate-Agarose?
1 answer-
Since N6-[N-(6-aminohexyl)-carbamyl]-ADP is a substrate for acetate kinase and pyruvate kinase, these enzymes can be used to regenerate ATP from ADP on the resin under conditions favoring the reverse reaction. Wash the resin with 20 volumes of 50 mM Tris HCl buffer (pH 8.2), with 0.2 mM EDTA and 100 mM KCl. Incubate the resin overnight at 2-8°C in an ATP-regenerating mixture containing 0.2 mM phosphoenol pyruvate, pyruvate kinase (10 units per mL of resin), 5 mM MgCl2, 0.2 mM EDTA and 100 mM KCl in 50 mM Tris HCl buffer (pH 8.2). Wash the resin with 25 column volumes of 2 mM ATP in 2 M KCl and reequilibrate the resin with 25 column volumes of sample buffer. Alternatively, the ATP regenerating mixture can contain 20 mM acetyl phosphate, acetate kinase (6.8 units per mL of resin), and 3 mM MgCl2 in 100 mM Tris HCl (pH 7.6). If acetate kinase is used, prewash the resin with 25 column volumes of 100 mM Tris HCl (pH 7.6), but use the same final wash and reequilibration steps as given for pyruvate kinase.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I elute my proteins from Product A2767, Adenosine 5´-triphosphate-Agarose?
1 answer-
Specifically bound proteins can be eluted with 10-100 mM ATP or ADP. Nonspecifically bound proteins can be eluted with 2 M NaCl or KCl in water or 7 M urea.
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Can I store Product A2767, Adenosine 5´-triphosphate-Agarose, after I have hydrated it?
1 answer-
For the short term, it is possible to store the hydrated resin in 20% ethanol and dilute buffer at 2-8°C. In general, the hydrated resin can be stored refrigerated in water or buffer containing a bacteriostat, such as 0.02% sodium azide or thimerosal. Do not autoclave or freeze the hydrated resin. The resin can be used several times without loss of effectiveness. However, the ATP will slowly hydrolyze to ADP over time, and under certain conditions this process may be accelerated during usage. The resin can be regenerated; please see the regeneration FAQ for this product.
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