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重要文件

M1028

Sigma-Aldrich

氯化镁 溶液

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

同義詞:

MgCl2, dichloromagnesium, magnesium chloride

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About This Item

線性公式:
MgCl2
CAS號碼:
分子量::
95.21
MDL號碼:
分類程式碼代碼:
12352302
PubChem物質ID:
NACRES:
NA.52
等級:
for molecular biology
形狀:
liquid
溶解度:
water: soluble
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等級

for molecular biology

品質等級

無菌

non-sterile; 0.2 μm filtered

形狀

liquid

濃度

1.00 M±0.01 M

技術

DNA sequencing: suitable

雜質

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

溶解度

water: soluble

密度

1.080 g/cm3

正離子痕跡

heavy metals (as Pb): ≤5 ppm

適合性

suitable for electrophoresis
suitable for molecular biology

應用

cell analysis

異物活動

DNase, RNase, NICKase and protease, none detected

SMILES 字串

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI 密鑰

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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一般說明

氯化镁是一种常见的离子型卤化物,易溶于水。它在酶学、细胞膜/细胞壁结构、肌肉细胞生理学和核酸结构中起多种重要的生物学作用。此外,氯化镁在许多酶中都是基本的辅酶,包括脱氧核糖核酸酶、某些限制性酶和核糖核酸酶H。氯化镁在各种分子生物学应用中(包括PCR反应)广泛用于提供镁离子。

應用

氯化镁在各种分子生物学应用,包括PCR反应和缓冲液中提供镁离子。用途:
  • 配制免疫沉淀实验的细胞裂解缓冲液[1]
  • 加入Krebs-Henseleit缓冲液,用于老年小鼠的长期高脂饮食研究。[2]
  • 用于三维微生物转谷氨酰胺酶水凝胶中的细胞透化,进行骨细胞分化及互连细胞网络的体外研究。[3]
  • 保护肝脏免遭氧化损伤。[4]
  • 制备无血清培养基,用于大鼠脑切片培养物的电生理研究。[5]

特點和優勢

  • 即用型溶液,免去配制步骤,节约时间。
  • 重金属(以铅计):≤5 ppm
  • 不含DNA酶、RNA酶、切口酶和蛋白酶

成分

氯化镁溶液是含有1M氯化镁的18兆欧水。

其他說明

如需了解生化试剂系列的更多信息,请填写此表

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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Protective Effects of Magnesium Chloride on Liver Enzymes and Biomarkers of Oxidative Stress in high fat diet fed Rats
Mohammed et, al
Nigerian Journal of Physiological Sciences : Official Publication of the Physiological Society of Nigeria, 34(2), 149-157 (2019)
M J Mc Garrigle et al.
European cells & materials, 31, 323-340 (2016-05-25)
Extracellular matrix (ECM) stiffness and cell density can regulate osteoblast differentiation in two dimensional environments. However, it is not yet known how osteoblast-osteocyte differentiation is regulated within a 3D ECM environment, akin to that existing in vivo. In this study
Yelena Y Grinberg et al.
Journal of neurochemistry, 122(1), 221-229 (2012-04-25)
Spreading depression (SD), the likely cause of migraine aura and perhaps migraine, is triggered by widespread and unfettered neuronal hyperexcitability. Migraine and the initiating hyperexcitability of seizure, which involve oxidative stress (OS), are likely interrelated. Environmental enrichment (EE) decreases seizure
Antimo Migliaccio et al.
Methods in molecular biology (Clifton, N.J.), 776, 361-370 (2011-07-29)
Much evidence indicates that, with few exceptions, non-genomic actions of steroids are mediated by receptors universally known as nuclear receptors. Steroid receptors do not exhibit intrinsic tyrosine kinase activity. Nevertheless, they stimulate different signaling pathways in cytoplasm of target cells
Hiroaki Eshima et al.
Journal of applied physiology (Bethesda, Md. : 1985), 128(5), 1153-1162 (2020-03-28)
Obesity and aging reduce skeletal muscle contractile function. However, it remains unclear whether obesity additively promotes muscle contractile dysfunction in the setting of aging. In this study, we investigated skeletal muscle contractile function ex vivo and intracellular Ca2+ release in

條款

This procedure may be used for Mutanolysin products.

在测定变溶菌素活性时,使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。

在测定己糖激酶活性时,使用了一种在340nm处的连续分光光度法进行测定。己糖激酶使用ATP作为磷酸的来源来催化D-己糖的磷酸化。

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

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