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重要文件

83636

Sigma-Aldrich

Resorufin acetate

suitable for fluorescence, ≥98.0% (TLC)

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About This Item

經驗公式(希爾表示法):
C14H9NO4
CAS號碼:
分子量::
255.23
Beilstein:
246139
MDL號碼:
分類程式碼代碼:
12352200
PubChem物質ID:
NACRES:
NA.32

化驗

≥98.0% (TLC)

形狀

powder or crystals

mp

223 °C (lit.)

溶解度

DMF: soluble
DMSO: soluble
alcohols: soluble

螢光

λex 500 nm; λem 593 nm in 0.1 M phosphate pH 8.0 (chymotrypsin)
λex 540 nm; λem 590 nm in DMSO
λex 571 nm; λem 584 nm (Reaction product)

適合性

suitable for fluorescence

儲存溫度

2-8°C

SMILES 字串

CC(=O)Oc1ccc2N=C3C=CC(=O)C=C3Oc2c1

InChI

1S/C14H9NO4/c1-8(16)18-10-3-5-12-14(7-10)19-13-6-9(17)2-4-11(13)15-12/h2-7H,1H3

InChI 密鑰

UJWKHDKBOVPINX-UHFFFAOYSA-N

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應用

suitable as fluorogenic substrate for hydrolytic enzymes (cellulases, chymotrypsin)

其他說明

Studies on the esterase activity of cytosolic aldehyde dehydrogenase

象形圖

Exclamation mark

訊號詞

Warning

危險分類

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


分析證明 (COA)

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Myung Gil Choi et al.
Organic letters, 12(7), 1468-1471 (2010-03-13)
The acetate derivatives of fluorescein and resorufin exhibited a prominent turn-on type signaling behavior toward BO(3)(-) ions over other common anions. Signaling is based on the selective deprotection of acetate groups by perborate, which resulted in significant chromogenic and fluorogenic
The action of cytosolic aldehyde dehydrogenase on resorufin acetate.
T M Kitson et al.
Advances in experimental medicine and biology, 414, 201-208 (1997-01-01)
T M Kitson
Biochimica et biophysica acta, 1385(1), 43-52 (1998-06-19)
Resorufin bromoacetate is a substrate that is rapidly hydrolysed by chymotrypsin. The reaction shows a pre-steady-state burst phase that may be observed by stopped flow spectrophotometry if precautions are taken against spontaneous hydrolysis of the substrate. The strongly activating effect
T M Kitson et al.
The Biochemical journal, 322 ( Pt 3), 701-708 (1997-03-15)
Resorufin acetate is a very good substrate for sheep liver cytosolic aldehyde dehydrogenase, both from the point of view of practical spectrophotometry and in terms of information provided about the nature of the catalysis shown by this enzyme. p-Nitrophenyl (PNP)
Mohit P Mathew et al.
Chembiochem : a European journal of chemical biology, 18(13), 1204-1215 (2017-02-22)
This report describes the metabolic glycoengineering (MGE) of intracellular esterase activity in human colon cancer (LS174T) and Chinese hamster ovary (CHO) cells. In silico analysis of carboxylesterases CES1 and CES2 suggested that these enzymes are modified with sialylated N-glycans, which

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