This product is an aqueous solution containing 35 - 55% glycerol.
76216
木瓜蛋白酶 来源于番木瓜
solution, light brown, ≥10 U/mg protein (~25 mg/ml)
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CAS 編號:
UNSPSC Code:
12352204
eCl@ss:
42030311
EC Number:
232-627-2
NACRES:
NA.54
MDL number:
Specific activity:
≥10 U/mg protein (~25 mg/ml)
Biological source:
papaya
跳轉至
biological source
papaya
form
solution
specific activity
≥10 U/mg protein (~25 mg/ml)
Quality Level
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
color
light brown
storage temp.
2-8°C
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本產品 | |||
|---|---|---|---|
| specific activity ≥10 U/mg protein (~25 mg/ml) | specific activity ≥8.0 units/mg protein | specific activity 30 U/mg, ~30 units/mg protein (At 25 °C with BAEE as the substrate.) | specific activity ≥10 units/mg protein |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) - | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable |
| biological source papaya | biological source papaya (latex) | biological source papaya | biological source papaya (latex) |
| form solution | form lyophilized powder | form suspension | form lyophilized powder |
| mol wt Mr ~23000 | mol wt 23.4 kDa | mol wt 23 kDa | mol wt 23.4 kDa |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. - | storage temp. −20°C |
General description
木瓜蛋白酶是一种天然存在于木瓜（Carica papaya L.）中的蛋白水解酶。可以从切开的未成熟木瓜的乳胶中获得。木瓜蛋白酶属于半胱氨酸蛋白酶家族。它是一种球状单链蛋白质，由四个二硫桥和催化基础残基组成。木瓜蛋白酶由全α-结构域和反平行片β-结构域组成。
Application
木瓜蛋白酶被用作嫩肉精。它还被用于在原代细胞培养物制备中分解组织。
Biochem/physiol Actions
木瓜蛋白酶是一种可切割肽键的半胱氨酸蛋白酶，并且已知可切割抗体的Fc部分。
木瓜蛋白酶对许多生物体的生物过程起重要作用。它对蛋白质、氨基酸酯和酰胺连接以及短链肽具有蛋白水解活性。木瓜蛋白酶倾向于切割碱性氨基酸如精氨酸、苯丙氨酸和赖氨酸的肽键。它在药物发现、制药、食品、烘焙和乳制品行业有广泛的应用。
Other Notes
1 U对应于在pH 6.2和25°C下，每分钟水解1 μmol N-苯甲酰基-L-精氨酸乙酯（BAEE，货号 12880）所需的酶量
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存儲類別/等級
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Immobilization of papain: A review
Veymar G, et al.
International Journal of Biological Macromolecules, 188, 94-113 (2021)
The effect of glycosaminoglycan enzymes and proteases on the viscosity of alpaca seminal plasma and sperm function
Kershaw CM, et al.
Animal Reproduction Science, 138(1-2), 261-267 (2013)
Papain , a plant enzyme of biological importance: a review
Mamboya E.
American Journal of Biochemistry and Biotechnology, 8(2), 99-104 (2012)
José M Laparra et al.
Journal of food science, 74(6), H168-H173 (2009-09-03)
This study aims to understand the enhancing effect of glycosaminoglycans (GAGs), such as chondroitin/dermatan structures, on Fe uptake to Caco-2 cells. High-sulfated GAGs were selectively purified from cooked haddock. An in vitro digestion/Caco-2 cell culture model was used to evaluate
Extraction of Near-Field Fluorescence from Composite Signals to Provide High Resolution Images of Glial Cells
Robert T D, et al.
Biophysical Journal, 80(5), 2477-2482 (2001)
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Which solution is used in Papain from Carica papaya solution? DMSO? water?
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