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52283

Sigma-Aldrich

Anti-Mouse IgG−Abberior® STAR RED antibody produced in goat

for STED application

同義詞:

Abberior® STAR RED-Anti-Mouse-IgG antibody produced in goat

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.45

生物源

goat

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

mouse

濃度

~1 mg/mL

螢光

λex 638 nm; λem 655 nm in PBS, pH 7.4

儲存溫度

−20°C

一般說明

Abberior STAR RED is a well-known red dye, named also KK114 in literature. It was developed for STED and confocal imaging in the red spectral region. It shows an exceptional brightness and low background, delivering stunning STED images. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. Abberior STAR RED can substitute dyes like Atto 647N, Alexa Fluor 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Abberior STAR RED is the ideal partner for Abberior STAR 580 to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 125 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,16 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,32 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 654 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,4 (PBS pH 7.4)

適合性

Designed and tested for fluorescent super-resolution microscopy

分析報告

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

法律資訊

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Maksim V Sednev et al.
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The synthesis, reactivity, and photophysical properties of new rhodamines with intense red fluorescence, two polar residues (hydroxyls, primary phosphates, or sulfonic acid groups), and improved hydrolytic stability of the amino-reactive sites (NHS esters or mixed N-succinimidyl carbonates) are reported. All
Erdinc Sezgin et al.
Biochimica et biophysica acta, 1818(7), 1777-1784 (2012-03-28)
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