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Merck
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文件

30558

Sigma-Aldrich

Abberior® STAR 635, NHS ester

for STED application

同義詞:

super-resolution Microscopy

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About This Item

分類程式碼代碼:
12352108
NACRES:
NA.32

濃度

≥50.0% (degree of coupling)

溶解度

DMF: 0.25 mg/mL, clear

螢光

λex 635 nm; λem 655 nm±10 nm in PBS, pH 7.4

儲存溫度

−20°C

一般說明

Absorption Maximum, λmax: 635 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 110,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.26 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.38 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 656 nm (aq. acetonitrile; MeOH)
655 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 −780 nm
Fluorescence Quantum Yield, η: 0.88 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 2.8 (PBS, pH 7.4)

應用

  • Abberior STAR 635 goat anti-rabbit antibody has been used for STED (stimulated emission depletion) imaging of primary cultured striatal neurons obtained from rats.
  • Abberior STAR 635 anti-rabbit antibody has been used for STED imaging of non-sensory supporting cells obtained from cochlea of rats and mice.
  • Abberior STAR 635 conjugated with secondary antibody has been used for STED imaging of microtubules in cells.

適合性

Designed and tested for fluorescent super-resolution microscopy

法律資訊

6538 is a trademark of American Type Culture Collection
abberior is a registered trademark of Abberior GmbH

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Hans Blom et al.
PloS one, 8(9), e75155-e75155 (2013-09-24)
The phosphoprotein DARPP-32 (dopamine and cyclic adenosine 3´, 5´-monophosphate-regulated phosphoprotein, 32 kDa) is an important component in the molecular regulation of postsynaptic signaling in neostriatum. Despite the importance of this phosphoprotein, there is as yet little known about the nanoscale
Jenu Varghese Chacko et al.
Journal of biomedical optics, 19(10), 105003-105003 (2014-10-08)
Atomic force microscopes (AFM) provide topographical and mechanical information of the sample with very good axial resolution, but are limited in terms of chemical specificity and operation time-scale. An optical microscope coupled to an AFM can recognize and target an
Arnaud P Giese et al.
Development (Cambridge, England), 139(20), 3775-3785 (2012-09-20)
Vangl2 is one of the central proteins controlling the establishment of planar cell polarity in multiple tissues of different species. Previous studies suggest that the localization of the Vangl2 protein to specific intracellular microdomains is crucial for its function. However
T A Klar et al.
Optics letters, 24(14), 954-956 (2007-12-13)
We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited
Stefan W Hell
Nature biotechnology, 21(11), 1347-1355 (2003-11-05)
For more than a century, the resolution of focusing light microscopy has been limited by diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently, microscopes have been reported that provide three- to

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