This product may be stored at room temperature.
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等級
Molecular Biology
for molecular biology
蒸汽密度
1.55 (vs air)
蒸汽壓力
0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)
產品線
BioUltra
化驗
≥99.5% (T)
形狀
liquid
自燃溫度
932 °F
技術
FISH: suitable
hybridization: suitable
protein extraction: suitable
雜質
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
燃燒殘留物
≤0.1%
折射率
n20/D 1.447 (lit.)
n20/D 1.447
pH值
7-9 (25 °C, 1 M in H2O)
bp
210 °C (lit.)
mp
2-3 °C (lit.)
溶解度
H2O: 10 M at 20 °C, clear, colorless
密度
1.134 g/mL at 25 °C (lit.)
負離子痕跡
chloride (Cl-): ≤1 mg/kg
sulfate (SO42-): ≤10 mg/kg
正離子痕跡
Ag: ≤5 mg/kg
Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
NH4+: ≤1000 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Tl: ≤5 mg/kg
Zn: ≤1 mg/kg
SMILES 字串
NC=O
&lambda ；
10 M in H2O
紫外吸收
λ: 260 nm Amax: ≤0.4
λ: 280 nm Amax: ≤0.1
應用
genomic analysis
InChI
1S/CH3NO/c2-1-3/h1H,(H2,2,3)
InChI 密鑰
ZHNUHDYFZUAESO-UHFFFAOYSA-N
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一般說明
甲酰胺，也称为甲酰胺醛，是由甲酸衍生出的酰胺化合物。它在生物化学研究中起着至关重要的作用，尤其是在核酸（RNA和DNA）的变性和复性方面。这包括杂交、测序凝胶电泳和电子显微镜等应用。在分子生物学中，BioUltra甲酰胺广泛用于多种应用，如核酸杂交缓冲液、基因指纹图谱、南部印迹杂交和北部印迹杂交，以及YAC筛选。它通常通过降低核酸杂交体的熔解温度来发挥作用，从而便于对其进行操作，并能在测序凝胶中改善变性条件，以获得更稳健的结果。
應用
甲酰胺可使核酸双螺旋不稳定，通常可以 50% 的浓度，用于需要较低杂交温度的杂交实验。
甲酰胺已用于：
- 作为用于原位杂交的杂交缓冲液中的组分，作为溶液 X 的组分，用于洗涤载玻片以去除盖玻片
- 光谱核型分析（SKY）的变性溶液的制备
- 比较基因组杂交（CGH）载玻片预处理的 1% 甲醛溶液的制备
- RNA 荧光原位杂交（RNA-FISH）的杂交前缓冲液 I、II、III、IV 和杂交缓冲液 I、II 和 III 的制备
- 作为生产甲酸酯和维生素的电离溶剂，纸张的软化剂，动物胶和水溶性胶，分子生物学中的变性剂
生化／生理作用
甲酰胺是极性有机和无机化合物的良好溶剂，由于其高介电常数，在益生元过程中具有潜在的催化活性。
特點和優勢
- 用途广泛，适应各种实验室和研究应用
- BioUltra级试剂，适合分子生物学、细胞生物学和生物化学研究
- 经检测确认，重金属污染水平低，适用性广
- 不含DNA酶、RNA酶、切口酶和蛋白酶检测了是否存在微量水平的阴离子和阳离子
訊號詞
Danger
危險聲明
危險分類
Carc. 2 - Repr. 1B - STOT RE 2 Oral
標靶器官
Blood
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 1
閃點（°F）
305.6 °F
閃點（°C）
152 °C
個人防護裝備
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
G. Wilkinson, ed.
In Situ Hybridization: A practical approach, 147-147 (1992)
R.C. Ogden et al.
Methods in Enzymology, 152, 67-67 (1987)
Min Chen et al.
Prenatal diagnosis, 35(6), 534-543 (2015-02-03)
To develop a single-tube multi-marker assay for improved preimplantation genetic diagnosis (PGD) of deletional and/or non-deletional Hb Bart's hydrops fetalis syndrome, providing haplotype confirmation of deletional status, and maximization of linkage informativity. We performed in silico mining to identify novel
A Touati et al.
Journal of clinical microbiology, 53(10), 3182-3194 (2015-07-24)
Molecular typing of Mycoplasma pneumoniae is an important tool for identifying grouped cases and investigating outbreaks. In the present study, we developed a new genotyping method based on single nucleotide polymorphisms (SNPs) selected from the whole-genome sequencing of eight M.
Jing Wang et al.
The journal of physical chemistry. B, 117(8), 2314-2320 (2013-01-26)
A step-by-step mechanistic pathway following the transformation of formamide to purine through a five-membered ring intermediate has been explored by density functional theory computations. The highlight of the mechanistic route detailed here is that the proposed pathway represents the simplest
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What temperature should this product be stored at?
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What temperature should this product be stored at?
1 answer-
This product may be stored at room temperature.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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What expiry does Formamide get?
1 answer-
The product specification sheet for this product contains a recommended retest date period of roughly 11 months after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the best way to deionize Formamide, Product 47671?
1 answer-
Mixed Bed Resin (e.g., Sigma Product No. M8157 or M8032) can be used to deionize formamide.
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What is the molarity of the neat liquid Formamide, Product 47671?
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Based on the density of 1.13 and the molecular weight of 45.04, the molarity of this neat liquid is 25.09 M. The pH of a 0.5 M aqueous solution is 7.1, according to the chemicals encyclopedia published by the Royal Society of Chemistry, 12th edition.
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Are aqueous solutions of Formamide, Product 47671, stable?
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In aqueous solution, formamide hydrolyzes to form ammonia and formic acid (i.e., ammonium formate).
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