M0178

Middlebrook 7H9 肉汤基础

suitable for microbiology, NutriSelect^® Plus,

• 同義詞: Mycobacterial culture media
• 分類程式碼代碼: 41171607
• NACRES: NA.85

屬性

• 品質等級: 200
• 無菌: non-sterile
• 產品線: BioChemika
• 形狀: powder
• 儲存期限: limited shelf life, expiry date on the label
• 製造商／商標名: NutriSelect^® Plus
• 技術: microbe id | susceptibility testing: suitable; microbiological culture: suitable
• 最終pH值: 6.6±0.2 (25 °C)
• 應用: clinical testing; food and beverages; life science and biopharma; veterinary; microbiology
• 儲存溫度: 2-25°C
• 適合性: mycobacteria

描述

一般說明

Middlebrook 7H9 Broth Base contains inorganic compounds that supply the elements potassium, sulfur, magnesium, and phosphorus which are required for appropriate growth of Mycobacteria. This medium with Middlebrook ADC Growth Supplement is recommended for ideal growth of Mycobacterium species.
Middlebrook ADC Growth Supplement (Product No. M0553) contains bovine albumin, dextrose, and catalase. Dextrose supplies energy, while albumin protects Mycobacteria from toxic agents and catalase splits toxic radicals.
Mycobacteria grow most rapidly in broth media and hence primary isolation of bacterial species can be carried out in Middlebrook 7H9 Broth Base.

應用

Middlebrook 7H9 Broth Base has been used for the cultivation Mycobacterium bovis bacillus Calmette-Guérin Pasteur strain. It is also recommended for cultivation and sensitivity testing of Mycobacterium tuberculosis.

成分

成分 (g/L)
硫酸铵，0.50
磷酸二钠，2.50
磷酸二氢钾，1.00
氯化钠，0.10
硫酸镁，0.05
氯化钙，0.0005
硫酸锌，0.001
硫酸铜，0.001
柠檬酸铁铵，0.04
L-谷氨酸，0.50
吡哆素，0.001
生物素，0.0005

準備報告

Suspend 2.35 g of Middlebrook 7H9 Broth Base in 450 ml of distilled water. Add either 2 ml of glycerol (Cat. No 49767) or 0.5 g of TWEEN^® 80 (Cat. No. 93780). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. Pressure (121°C) for 10 minutes. Cool to 45°C or below and aseptically add 1 vial of Middlebrook ADC Growth Supplement (Cat. No. M0553). Mix well before dispensing.

腳註

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法律資訊

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

安全資訊

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable