Toluidine Blue is used in various staining procedures, and preparation may vary depending on the specific procedure. For staining frozen sections, aqueous solutions with concentrations ranging from 0.3% to 1% can be used. Additionally, a 0.5% dye in 20% alcohol is suitable for frozen sections. Other solutions may require a specific pH or special preparation.
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About This Item
經驗公式（希爾表示法）:
C15H16ClN3S · 0.5ZnCl2
CAS號碼:
分子量：:
373.97
顏色索引編號:
52040
Beilstein:
4068052
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
MA.02
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等級
for microscopy (Hist., Vit.)
品質等級
化驗
8.74% (ZN (THEORY))
形狀
powder or crystals
技術
microbe id | staining: suitable
顏色
green to very dark green
溶解度
methanol: 0.01 g/10 mL, blue to very deep blue
εmax
≥800 at 621-636 nm in water
適合性
suitable for microscopy
應用
diagnostic assay manufacturing
hematology
histology
儲存溫度
room temp
SMILES 字串
[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C
InChI
1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2
InChI 密鑰
DAQXTSXITPUGRG-UHFFFAOYSA-L
一般說明
甲苯胺蓝（C.l.52040)是一种二氨基吩噻嗪染料。它也被称为TBO，托洛氯铵和甲苯胺蓝 O。
應用
甲苯胺蓝适用于以下应用：
- 证明淀粉样蛋白；植物材料，肥大细胞，尼氏物质、细胞核和硫苷脂中的DNA
- 和硫酸粘蛋白及相关物质的异色染色中的硫脂
- 作为染色剂鉴定软骨基质的发育和积累，蛋白多糖用于从小鼠密质骨中分离和培养间充质干细胞。
- 水溶性树脂中粘蛋白的染色和环氧树脂中核酸的染色(1)
生化／生理作用
甲苯胺蓝是一种碱性噻嗪异色染料，用于选择性染色酸性组织成分，如羧酸盐、硫酸盐和磷酸盐自由基。已发现它具有核酸亲和力，因此能与高DNA和RNA含量的组织的核物质结合。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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Are there any instructions on how to mix Toluidine Blue powder (89640) into a staining solution?
1 answer-
Helpful?
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What does C.l. 52040 mean?
1 answer-
The C.I. number is the Colour Index number. This number is globally recognized and is assigned to Dyes and Pigments by the Americal Association of Textile Chemists and Colourists (AATCC) established in 1924. This compound is assigned a C.I. number of 52040. Please see the link below to learn more about the Colour Index program:
https://colour-index.com/Helpful?
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