D-(+)-Melibiose is a reducing disaccharide composed of a unit of galactose linked to a unit of glucose by a glycosidic bond α (1 → 6). It is commonly found in plants and the roots of certain legumes. D-(+)-Melibiose is not metabolized by humans but serves as a substrate for certain bacteria and fungi. It is used as a differential medium component in microbiological media to detect the fermentation of melibiose by microorganisms. It helps identify specific bacterial species based on their ability to ferment melibiose.
應用
D-(+)-Melibiose has applications in the fields of microbiology, research and development, and in food and pharmaceutical industries. Melibiose fermentation test is used to differentiate bacteria like Enterobacteriaceae (like Salmonella, E.coli, shigella, Citrobacter, Klebsiella, Enterobacter) and yeasts. It is also used for the differentiation between the plant pathogenic bacteria Klebsiella pneumoniae and K. variicola.
儲存和穩定性
Keep the container tightly closed in a dry and well-ventilated place.Hygroscopic, Store under inert gas.
Comparative analysis of nucleotide sequences of genes participating in melibiose fermentation and isocitrate lyase production was conducted in 90 natural Yersinia pestis strains of main and non main subspecies. It was ascertained that the lack of the ability to utilize
Are melibiose-fermenting intestinal and alpechin strains of Saccharomyces cerevisiae a novel type of yeast probiotics?
G I Naumov
Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 439, 262-263 (2011-09-29)
Biochimica et biophysica acta, 1828(8), 1690-1699 (2013-03-19)
The melibiose transporter from Escherichia coli (MelB) can use the electrochemical energy of either H(+), Na(+) or Li(+) to transport the disaccharide melibiose to the cell interior. By using spectroscopic and biochemical methods, we have analyzed the role of Arg149
The Escherichia coli melR gene encodes the MelR transcription factor that controls melibiose utilization. Expression of melR is autoregulated by MelR, which represses the melR promoter by binding to a target that overlaps the transcript start. Here, we show that
Zhurnal mikrobiologii, epidemiologii, i immunobiologii, (4)(4), 75-81 (2010-08-28)
To characterize species specificity of officially recommended tests for differentiation of Yersiniapestis and Yersinia pseudotuberculosis and propose additional tests allowing for more accurate identification. Natural, laboratory and typical strains oftwo Yersinia species were studied using microbiological, molecular and biochemical methods.
Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.
